A hot pepper gene encoding WRKY transcription factor is induced during hypersensitive response to Tobacco mosaic virus and Xanthomonas campestris

Chang Jin Park, Yun Chul Shin, Boo Ja Lee, Ki Jeong Kim, Jeong Kook Kim, Kyung Hee Paek

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Plant WRKY transcription factors were previously implicated in the alteration of gene expression in response to various pathogens. The WRKY proteins constitute a large family of plant transcription factors, whose precise functions have yet to be elucidated. Using a domain-specific differential display procedure, we isolated a WRKY gene, which is rapidly induced during an incompatible interaction between hot pepper and Tobacco mosaic virus (TMV) or Xanthomonas campestris pv . vesicatoria (Xcv). The full-length cDNA of CaWRKY-a (Capsicum annuum WRKY-a) encodes a putative polypeptide of 546 amino acids, containing two WRKY domains with a zinc finger motif. The expression of CaWRKY-a could be rapidly induced by not only chemical elicitor such as salicylic acid (SA) or ethephon but also wounding treatments. The nuclear localization of CaWRKY-a was determined in transient expression system using tobacco BY-2 cells by polyethylene glycol (PEG)-mediated transformation experiment. With oligonucleotide molecules containing the putative W-box sequences as a probe, we confirmed that CaWRKY-a protein had W-box-binding activity. These results suggest that CaWRKY-a might be involved as a transcription factor in plant defense-related signal transduction pathways.

Original languageEnglish
Pages (from-to)168-179
Number of pages12
JournalPlanta
Volume223
Issue number2
DOIs
Publication statusPublished - 2006 Jan

Keywords

  • HH Xanthomonas campestris
  • Hot pepper (Capsicum annuum L.)
  • Hypersensitive response
  • Tobacco mosaic virus
  • WRKY transcription factor

ASJC Scopus subject areas

  • Genetics
  • Plant Science

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