A large mobility of hydrophilic molecules at the outmost layer controls the protein adsorption and adhering behavior with the actin fiber orientation of human umbilical vein endothelial cells (HUVEC)

Sachiro Kakinoki, Ji-Hun Seo, Yuuki Inoue, Kazuhiko Ishihara, Nobuhiko Yui, Tetsuji Yamaoka

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Adhesion behaviors of human umbilical vein endothelial cells (HUVECs) are interestingly affected by the mobility of hydrophilic chains on the material surfaces. Surfaces with different molecular mobilities were prepared using ABA-type block copolymers consisting polyrotaxane (PRX) or poly(ethylene glycol) (PEG) central block (A block), and amphiphilic anchoring B blocks of poly(2-methacryloyloxyethyl phosphorylcholine-co-n-butyl methacrylate) (PMB). Two different molecular mobilities of the PRX chains were designed by using normal α-cyclodextrin (α-CD) or α-CD whose hydroxyl groups were converted to methoxy groups in a given ratio to improve its molecular mobility (PRX-PMB and OMe-PRX-PMB). The surface mobility of these materials was assessed as the mobility factor (Mf), which is measured by quartz crystal microbalance with dissipation monitoring system. HUVECs adhered on OMe-PRX-PMB surface much more than PRX-PMB and PMB-block-PEG-block-PMB (PEG-PMB) surfaces. These different HUVEC adhesions were correlated with the density of cell-binding site of adsorbed fibronectin. In addition, the alignment of the actin cytoskeleton of adhered HUVECs was strongly suppressed on the PEG-PMB, PRX-PMB, and OMe-PRX-PMB in response to the increased Mf value. Remarkably, the HUVECs adhered on the OMe-PRX-PMB surface with much less actin organization. We concluded that not only the cell adhesion but also the cellular function are regulated by the molecular mobility of the outmost material surfaces.

Original languageEnglish
Pages (from-to)1320-1332
Number of pages13
JournalJournal of Biomaterials Science, Polymer Edition
Volume24
Issue number11
DOIs
Publication statusPublished - 2013 Aug 1
Externally publishedYes

Fingerprint

Endothelial cells
Human Umbilical Vein Endothelial Cells
Fiber reinforced materials
Adsorption
Actins
Proteins
Molecules
Polyethylene glycols
Ethylene Glycol
Cell adhesion
Cell Adhesion
Quartz crystal microbalances
Cyclodextrins
Quartz Crystal Microbalance Techniques
Binding sites
polyrotaxane
Block copolymers
Adhesion
Actin Cytoskeleton
Fibronectins

Keywords

  • cytoskeletal organization
  • HUVEC adhesion
  • polyrotaxane triblock copolymer
  • surface mobility

ASJC Scopus subject areas

  • Biophysics
  • Biomaterials
  • Bioengineering
  • Biomedical Engineering

Cite this

A large mobility of hydrophilic molecules at the outmost layer controls the protein adsorption and adhering behavior with the actin fiber orientation of human umbilical vein endothelial cells (HUVEC). / Kakinoki, Sachiro; Seo, Ji-Hun; Inoue, Yuuki; Ishihara, Kazuhiko; Yui, Nobuhiko; Yamaoka, Tetsuji.

In: Journal of Biomaterials Science, Polymer Edition, Vol. 24, No. 11, 01.08.2013, p. 1320-1332.

Research output: Contribution to journalArticle

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