A magnetically separable, highly stable enzyme system based on nanocomposites of enzymes and magnetic nanoparticles shipped in hierarchically ordered, mesocellular, mesoporous silica

Jungbae Kim, Jinwoo Lee, Hyon Bin Na, Byoung Chan Kim, Jong Kyu Youn, Ja Hun Kwak, Karam Moon, Eunwoong Lee, Jaeyun Kim, Jongnam Park, Alice Dohnalkova, Hyun Gyu Park, Man Bock Gu, Ho Nam Chang, Jay W. Grate, Taeghwan Hyeon

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

The development of a magnetically separable and highly stable enzyme system, using nanoparticles and mesoporous silica, was investigated. Nanometer-scale composites of enzyme molecules and hierarchically ordered magnetite nanoparticles were immobilized via a ship-in-a-bottle approach, which used co-adsorption of enzymes and magnetite (Fe3O4) nanoparticles. The enzyme molecules were crosslinked via glutaraldehyde (GA) treatment resulting in the effective entrapment of neighboring magnetite nanoparticles. These nanocomposites, called M-CLEAs, were found magnetically separable, highly loaded with enzymes, stable under harsh conditions, resistant to proteolytic digestion, and recyclable for iterative use with negligible loss of enzyme activity. The ship-in-a-bottle approach can be expanded to many other enzymes and has applications in bioremediation and bioconversion.

Original languageEnglish
Pages (from-to)1203-1207
Number of pages5
JournalSmall
Volume1
Issue number12
DOIs
Publication statusPublished - 2005 Dec 1

Fingerprint

Nanocomposites
Silicon Dioxide
Nanoparticles
Enzymes
Silica
Magnetite Nanoparticles
Magnetite nanoparticles
Bottles
Ships
Bioconversion
Molecules
Bioremediation
Enzyme activity
Glutaral
Environmental Biodegradation
Adsorption
Digestion
Composite materials

Keywords

  • Enzyme catalysis
  • Magnetic nanoparticles
  • Magnetic separation
  • Mesoporous materials
  • Silica

ASJC Scopus subject areas

  • Biomaterials
  • Engineering (miscellaneous)
  • Biotechnology
  • Medicine(all)

Cite this

A magnetically separable, highly stable enzyme system based on nanocomposites of enzymes and magnetic nanoparticles shipped in hierarchically ordered, mesocellular, mesoporous silica. / Kim, Jungbae; Lee, Jinwoo; Na, Hyon Bin; Kim, Byoung Chan; Youn, Jong Kyu; Kwak, Ja Hun; Moon, Karam; Lee, Eunwoong; Kim, Jaeyun; Park, Jongnam; Dohnalkova, Alice; Park, Hyun Gyu; Gu, Man Bock; Chang, Ho Nam; Grate, Jay W.; Hyeon, Taeghwan.

In: Small, Vol. 1, No. 12, 01.12.2005, p. 1203-1207.

Research output: Contribution to journalArticle

Kim, J, Lee, J, Na, HB, Kim, BC, Youn, JK, Kwak, JH, Moon, K, Lee, E, Kim, J, Park, J, Dohnalkova, A, Park, HG, Gu, MB, Chang, HN, Grate, JW & Hyeon, T 2005, 'A magnetically separable, highly stable enzyme system based on nanocomposites of enzymes and magnetic nanoparticles shipped in hierarchically ordered, mesocellular, mesoporous silica', Small, vol. 1, no. 12, pp. 1203-1207. https://doi.org/10.1002/smll.200500245
Kim, Jungbae ; Lee, Jinwoo ; Na, Hyon Bin ; Kim, Byoung Chan ; Youn, Jong Kyu ; Kwak, Ja Hun ; Moon, Karam ; Lee, Eunwoong ; Kim, Jaeyun ; Park, Jongnam ; Dohnalkova, Alice ; Park, Hyun Gyu ; Gu, Man Bock ; Chang, Ho Nam ; Grate, Jay W. ; Hyeon, Taeghwan. / A magnetically separable, highly stable enzyme system based on nanocomposites of enzymes and magnetic nanoparticles shipped in hierarchically ordered, mesocellular, mesoporous silica. In: Small. 2005 ; Vol. 1, No. 12. pp. 1203-1207.
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