A manganese superoxide dismutase (MnSOD) from Ruditapes philippinarum: Comparative structural- and expressional-analysis with copper/zinc superoxide dismutase (Cu/ZnSOD) and biochemical analysis of its antioxidant activities

Navaneethaiyer Umasuthan, S. D N K Bathige, Kasthuri Saranya Revathy, Youngdeuk Lee, Ilson Whang, Cheol Young Choi, Hae Chul Park, Jehee Lee

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Superoxide dismutases (SODs), antioxidant metalloenzymes, represent the first line of defense in biological systems against oxidative stress caused by excessive reactive oxygen species (ROS), in particular O2•- Two distinct members of SOD family were identified from Manila clam Ruditapes philippinarum (abbreviated as RpMnSOD and RpCu/ZnSOD). The structural analysis revealed all common characteristics of SOD family in both RpSODs from primary to tertiary levels, including three MnSOD signatures and two Cu/ZnSOD signatures as well as invariant Mn2+- and Cu/Zn2+-binding sites in RpMnSOD and RpCu/ZnSOD, respectively. Putative RpMnSOD and RpCu/ZnSOD proteins were predicted to be localized in mitochondrial matrix and cytosol, respectively. They shared 65.2% and 63.9% of identity with human MnSOD and Cu/ZnSOD, respectively. Phylogentic evidences indicated the emergence of RpSODs within molluscan monophyletic clade. The analogous spatial expression profiles of RpSODs demonstrated their higher mRNA levels in hemocytes and gills. The experimental challenges with poly I:C, lipopolysaccharide and Vibrio tapetis illustrated the time-dependent dynamic expression of RpSODs in hemocytes and gills. The recombinant RpMnSOD was expressed in a prokaryotic system and its antioxidant property was studied. The rRpMnSOD exhibited its optimum activity at 20 °C, under alkaline condition (pH 9) with a specific activity of 3299 U mg-1. These outcomes suggested that RpSODs were constitutively expressing inducible proteins that might play crucial role(s) in innate immunity of Manila clam.

Original languageEnglish
Pages (from-to)753-765
Number of pages13
JournalFish and Shellfish Immunology
Volume33
Issue number4
DOIs
Publication statusPublished - 2012 Oct 1

Fingerprint

Ruditapes philippinarum
antioxidant
Superoxide Dismutase
manganese
Zinc
Copper
superoxide dismutase
Antioxidants
antioxidant activity
copper
zinc
protein
structural analysis
immunity
hemocytes
gills
Vibrio tapetis
matrix
antioxidants
polyinosinic-polycytidylic acid

Keywords

  • Antioxidant activity
  • Copper/znic superoxide dimutase (Cu/ZnSOD)
  • Gene expression
  • Manganese superoxide dismutase (MnSOD)
  • Ruditapes philippinarum

ASJC Scopus subject areas

  • Aquatic Science
  • Environmental Chemistry

Cite this

A manganese superoxide dismutase (MnSOD) from Ruditapes philippinarum : Comparative structural- and expressional-analysis with copper/zinc superoxide dismutase (Cu/ZnSOD) and biochemical analysis of its antioxidant activities. / Umasuthan, Navaneethaiyer; Bathige, S. D N K; Revathy, Kasthuri Saranya; Lee, Youngdeuk; Whang, Ilson; Choi, Cheol Young; Park, Hae Chul; Lee, Jehee.

In: Fish and Shellfish Immunology, Vol. 33, No. 4, 01.10.2012, p. 753-765.

Research output: Contribution to journalArticle

Umasuthan, Navaneethaiyer ; Bathige, S. D N K ; Revathy, Kasthuri Saranya ; Lee, Youngdeuk ; Whang, Ilson ; Choi, Cheol Young ; Park, Hae Chul ; Lee, Jehee. / A manganese superoxide dismutase (MnSOD) from Ruditapes philippinarum : Comparative structural- and expressional-analysis with copper/zinc superoxide dismutase (Cu/ZnSOD) and biochemical analysis of its antioxidant activities. In: Fish and Shellfish Immunology. 2012 ; Vol. 33, No. 4. pp. 753-765.
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AU - Bathige, S. D N K

AU - Revathy, Kasthuri Saranya

AU - Lee, Youngdeuk

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