A mast cell degranulation screening assay for the identification of novel mast cell activating agents

Herman F. Staats, Shaun M. Kirwan, Hae Woong Choi, Christopher P. Shelburne, Soman N. Abraham, Gulice Y.C. Leung, David Y.K. Chen

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

The development and use of vaccines and their ability to prevent infection/disease is a shining example of the benefit of biomedical research. Modern vaccines often utilize subunit immunogens that exhibit minimal immunogenicity and require the use of adjuvants to maximize the induction of protective immune responses. We recently described a novel class of vaccine adjuvants, mast cell (MC) activators, that exhibit safe and effective vaccine adjuvant activity when administered by intranasal or intradermal routes. A compound library containing 580 functionalized benzopyrans, a structural motif found in a diverse array of natural and designed bioactive compounds, was screened using a MC degranulation assay to identify novel MC activating compounds for future evaluation as novel vaccine adjuvants. This approach identified 12 novel MC degranulating compounds. Therefore, MC degranulation can be used to reliably detect novel compounds for evaluation as adjuvants for use in mucosal vaccine strategies.

Original languageEnglish
Pages (from-to)88-94
Number of pages7
JournalMedChemComm
Volume4
Issue number1
DOIs
Publication statusPublished - 2013 Jan
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Organic Chemistry

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