A metabolomic approach to determine the geographical origins of Anemarrhena asphodeloides by using UPLC-QTOF MS

Nahyun Kim, Seung Mok Ryu, DongHyuk Lee, Jae Won Lee, Eun Kyoung Seo, Je Hyun Lee, Dongho Lee

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

An ultraperformance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF MS) method was developed for metabolite profiling of Anemarrhena asphodeloides Bunge from two different geographical origins. In this study, the metabolite profile data obtained using UPLC-QTOF MS was subjected to multivariate statistical analyses, such as the principal component analysis and the hierarchical clustering analysis, to compare metabolite patterns among A. asphodeloides samples. Furthermore, a metabolite selection method known as significance analysis of microarrays (SAM) was applied to further select metabolites and to identify key constituents to efficiently distinguish between geographical origins. The UPLC-QTOF MS analysis successfully classified 21 samples into two distinct groups according to their geographical origins. The validation method used to assess the analytical stability and accuracy of these data is also described. These results suggest that this proposed method is reliable, accurate, and effective for geographic classification of A. asphodeloides, thus guiding its proper use for therapeutic purposes.

Original languageEnglish
Pages (from-to)47-52
Number of pages6
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume92
DOIs
Publication statusPublished - 2014 Apr 15

Fingerprint

Anemarrhena
Metabolomics
Liquid chromatography
Metabolites
Liquid Chromatography
Mass spectrometry
Mass Spectrometry
Therapeutic Uses
Microarray Analysis
Principal Component Analysis
Cluster Analysis
Microarrays
Multivariate Analysis
Principal component analysis

Keywords

  • Anemarrhena asphodeloides
  • Metabolite profiling
  • Metabolite selection
  • Method validation
  • UPLC-QTOF MS

ASJC Scopus subject areas

  • Analytical Chemistry
  • Drug Discovery
  • Pharmaceutical Science
  • Spectroscopy
  • Clinical Biochemistry

Cite this

A metabolomic approach to determine the geographical origins of Anemarrhena asphodeloides by using UPLC-QTOF MS. / Kim, Nahyun; Ryu, Seung Mok; Lee, DongHyuk; Lee, Jae Won; Seo, Eun Kyoung; Lee, Je Hyun; Lee, Dongho.

In: Journal of Pharmaceutical and Biomedical Analysis, Vol. 92, 15.04.2014, p. 47-52.

Research output: Contribution to journalArticle

Kim, Nahyun ; Ryu, Seung Mok ; Lee, DongHyuk ; Lee, Jae Won ; Seo, Eun Kyoung ; Lee, Je Hyun ; Lee, Dongho. / A metabolomic approach to determine the geographical origins of Anemarrhena asphodeloides by using UPLC-QTOF MS. In: Journal of Pharmaceutical and Biomedical Analysis. 2014 ; Vol. 92. pp. 47-52.
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