A method for the separation of mouse pancreatic islets using discontinuous Percoll gradient centrifugation

Yu Ree Cho, Sangduk Kim, Hyo Ihl Chang, Ha Chin Sung, Cherl Ho Lee, Chan Wha Kim

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A discontinuous Percoil gradient was used to separate islets from the collagenase-treated mouse pancreas easily and rapidly. Since the osmolality of Percoll is very low, adjustment of its osmolality to 340 mOs/kg H2O was essential for securing the optimal separation. A discontinuous gradient layering with Percoll solution of 1.09 g, 1.07g, and 1.05 g/ml, respectively, when centrifuged at 800 xg for 10 min, resulted in an optimal condition for separation and yielded a banding pattern with an even distribution of islet cells. No significant difference was observed in the morphological features between the Percoll-isolated and the manually-isolated islets. In conclusion, the discontinuous Percoll gradient can be effectively used to isolate the pancreatic islets from mice with four-fold higher efficiency compared to the handpicking method.

Original languageEnglish
Pages (from-to)522-524
Number of pages3
JournalJournal of microbiology and biotechnology
Volume9
Issue number4
Publication statusPublished - 1999 Aug

Keywords

  • Gradient centrifugation
  • Osmolality
  • Pancreatic islet
  • Percoll

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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