A method for the separation of mouse pancreatic islets using discontinuous Percoll gradient centrifugation

Yu Ree Cho, Sangduk Kim, Hyo-Ihl Chang, Ha Chin Sung, Cherl Ho Lee, Chan Wha Kim

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A discontinuous Percoil gradient was used to separate islets from the collagenase-treated mouse pancreas easily and rapidly. Since the osmolality of Percoll is very low, adjustment of its osmolality to 340 mOs/kg H2O was essential for securing the optimal separation. A discontinuous gradient layering with Percoll solution of 1.09 g, 1.07g, and 1.05 g/ml, respectively, when centrifuged at 800 xg for 10 min, resulted in an optimal condition for separation and yielded a banding pattern with an even distribution of islet cells. No significant difference was observed in the morphological features between the Percoll-isolated and the manually-isolated islets. In conclusion, the discontinuous Percoll gradient can be effectively used to isolate the pancreatic islets from mice with four-fold higher efficiency compared to the handpicking method.

Original languageEnglish
Pages (from-to)522-524
Number of pages3
JournalJournal of Microbiology and Biotechnology
Volume9
Issue number4
Publication statusPublished - 1999 Aug 1

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Centrifugation
Islets of Langerhans
Osmolar Concentration
Collagenases
Pancreas
Percoll

Keywords

  • Gradient centrifugation
  • Osmolality
  • Pancreatic islet
  • Percoll

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

A method for the separation of mouse pancreatic islets using discontinuous Percoll gradient centrifugation. / Cho, Yu Ree; Kim, Sangduk; Chang, Hyo-Ihl; Sung, Ha Chin; Lee, Cherl Ho; Kim, Chan Wha.

In: Journal of Microbiology and Biotechnology, Vol. 9, No. 4, 01.08.1999, p. 522-524.

Research output: Contribution to journalArticle

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