A micellar complex of a conjugated polyelectrolyte for efficient FRET to dye-labeled DNA

Rati Ranjan Nayak, Okhil Kumar Nag, Mijeong Kang, Youngeup Jin, Hongsuk Suh, Kwanghee Lee, Han Young Woo

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

A polymer-surfactant micellar complex has been studied as a fluorescence resonance energy transfer (FRET) donor to fluorescein-labeled DNA (ssDNA-Fl). In water, the molar absorptivity and fluorescence quantum efficiency of cationic poly(fluorene-co-phenylene) (c-PFP) are substantially increased in the presence of non-ionic surfactants. A TEM microscopic study shows the formation of a nanowire micellar complex of c-PFP and the surfactants. About a 400% enhancement of the FRET signal is measured in c-PFP/ssDNA-Fl with Brij 30, relative to that without surfactants. The signal amplification is successfully modulated using different types of non-ionic surfactants which perturb the complexation, fine-structure of the complex (i.e., donor-acceptor separation), and the resulting energy transfer process.

Original languageEnglish
Pages (from-to)633-638
Number of pages6
JournalMacromolecular Rapid Communications
Volume30
Issue number8
DOIs
Publication statusPublished - 2009 Apr 20
Externally publishedYes

Keywords

  • Conjugated polyelectrolytes
  • Donor-acceptor systems
  • FRET
  • Fluorescence
  • Surfactants

ASJC Scopus subject areas

  • Organic Chemistry
  • Polymers and Plastics
  • Materials Chemistry

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