A microfabricated reservoir-type oxygen sensor for measuring the real-time cellular oxygen consumption rate at various conditions

Jungil Park, Youngmi Kim Pak, James Jungho Pak

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

This paper presents a microfabricated reservoir-type oxygen sensor, which can accurately measure the solubilized oxygen concentration in real time, in order to measure the cellular oxygen consumption rate (OCR) in a solution containing cells. The fabricated oxygen sensor is composed of three-parts: electrochemical sensing electrodes, an oxygen-permeable membrane, and a reservoir for storing the solution. The oxygen transport rate through the membrane and the oxygen reaction rate at the working electrode (WE) surface are the two dominant parameters in determining the sensitivity of the oxygen sensor. The fabricated sensor showed a sensitivity of 2.84 A/cm2 M and a 90% response time of 4.9 s in an average of 5 sensors when a 25,000 μm2 WE and a 20 μm polydimethylsiloxane membrane were used. This is the first report in which the fastest response time has been achieved for the oxygen sensor. The fabricated sensor showed the repeatability with 154.05 ± 1.87 nA at the full-oxygen state and 2.77 ± 1.0 nA at the zero-oxygen state. The fabricated sensor was used to measure the uncoupled OCR of the L6 cells, and its result of 3.69 ± 0.30 was almost identical to the result of 3.70 ± 0.26 obtained from a commercial system.

Original languageEnglish
Pages (from-to)263-269
Number of pages7
JournalSensors and Actuators, B: Chemical
Volume147
Issue number1
DOIs
Publication statusPublished - 2010 May 18

Keywords

  • Cellular respiration
  • Electrochemistry
  • Oxygen consumption rate
  • Oxygen sensor
  • Reservoir-type

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

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