Abstract
We report a microfluidic array for investigating and quantitatively analyzing human neural stem cell (hNSC) self-renewal and differentiation in an invivo-like microenvironment. NSC niche conditions, including three-dimensional (3D) extracellular matrices and low oxygen tension, were effectively reconstituted in the microfluidic array in a combinatorial manner. The array device was fabricated to be detachable, rendering it compatible with quantitative real-time polymerase chain reaction for quantifying the effects of the biomimetic conditions on hNSC self-renewal and differentiation. We show that throughput of 3D cell culture and quantitative analysis can be increased. We also show that 3D hypoxic microenvironments maintain hNSC self-renewal capacity and direct neuronal commitment during hNSC differentiation.
| Original language | English |
|---|---|
| Pages (from-to) | 6607-6614 |
| Number of pages | 8 |
| Journal | Biomaterials |
| Volume | 34 |
| Issue number | 28 |
| DOIs | |
| Publication status | Published - 2013 Sep 1 |
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Keywords
- Differentiation
- Hypoxia
- Microfluidic array
- Neural stem cells
- Self-renewal
ASJC Scopus subject areas
- Biomaterials
- Bioengineering
- Ceramics and Composites
- Mechanics of Materials
- Biophysics
Cite this
A microfluidic array for quantitative analysis of human neural stem cell self-renewal and differentiation in three-dimensional hypoxic microenvironment. / Yang, Kisuk; Han, Sewoon; Shin, Yoojin; Ko, Eunkyung; Kim, Jin; Park, Kook In; Chung, Seok; Cho, Seung Woo.
In: Biomaterials, Vol. 34, No. 28, 01.09.2013, p. 6607-6614.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A microfluidic array for quantitative analysis of human neural stem cell self-renewal and differentiation in three-dimensional hypoxic microenvironment
AU - Yang, Kisuk
AU - Han, Sewoon
AU - Shin, Yoojin
AU - Ko, Eunkyung
AU - Kim, Jin
AU - Park, Kook In
AU - Chung, Seok
AU - Cho, Seung Woo
PY - 2013/9/1
Y1 - 2013/9/1
N2 - We report a microfluidic array for investigating and quantitatively analyzing human neural stem cell (hNSC) self-renewal and differentiation in an invivo-like microenvironment. NSC niche conditions, including three-dimensional (3D) extracellular matrices and low oxygen tension, were effectively reconstituted in the microfluidic array in a combinatorial manner. The array device was fabricated to be detachable, rendering it compatible with quantitative real-time polymerase chain reaction for quantifying the effects of the biomimetic conditions on hNSC self-renewal and differentiation. We show that throughput of 3D cell culture and quantitative analysis can be increased. We also show that 3D hypoxic microenvironments maintain hNSC self-renewal capacity and direct neuronal commitment during hNSC differentiation.
AB - We report a microfluidic array for investigating and quantitatively analyzing human neural stem cell (hNSC) self-renewal and differentiation in an invivo-like microenvironment. NSC niche conditions, including three-dimensional (3D) extracellular matrices and low oxygen tension, were effectively reconstituted in the microfluidic array in a combinatorial manner. The array device was fabricated to be detachable, rendering it compatible with quantitative real-time polymerase chain reaction for quantifying the effects of the biomimetic conditions on hNSC self-renewal and differentiation. We show that throughput of 3D cell culture and quantitative analysis can be increased. We also show that 3D hypoxic microenvironments maintain hNSC self-renewal capacity and direct neuronal commitment during hNSC differentiation.
KW - Differentiation
KW - Hypoxia
KW - Microfluidic array
KW - Neural stem cells
KW - Self-renewal
UR - http://www.scopus.com/inward/record.url?scp=84879461661&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84879461661&partnerID=8YFLogxK
U2 - 10.1016/j.biomaterials.2013.05.067
DO - 10.1016/j.biomaterials.2013.05.067
M3 - Article
C2 - 23777909
AN - SCOPUS:84879461661
VL - 34
SP - 6607
EP - 6614
JO - Biomaterials
JF - Biomaterials
SN - 0142-9612
IS - 28
ER -