A microfluidic-based bubble generation platform enables analysis of physical property change in phospholipid surfactant layers by interfacial ozone reaction

Young Shik Shin, Tae Su Choi, Hyungjun Kim, J. L. Beauchamp, James R. Heath, Hugh I. Kim

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The air-liquid interface filled with pulmonary surfactant is a unique feature of our lung alveoli. The mechanical properties of this interface play an important role in breathing and its malfunction induced by an environmental hazard, such as ozone, relates to various lung diseases. In order to understand the interfacial physics of the pulmonary surfactant system, we employed a microfluidic bubble generation platform with a model pulmonary surfactant composed of two major phospholipids: DPPC (1,2-dipalmitoyl-sn- phosphatidylcholine) and POPG (1-palmitoyl-2-oleoyl-sn-phosphatidylglycerol). With fluorescence imaging, we observed the ozone-induced chemical modification of the unsaturated lipid component of the lipid mixture, POPG. This chemical change due to the oxidative stress was further utilized to study the physical characteristics of the interface through the bubble formation process. The physical property change was evaluated through the oscillatory behaviour of the monolayer, as well as the bubble size and formation time. The results presented demonstrate the potential of this platform to study interfacial physics of lung surfactant system under various environmental challenges, both qualitatively and quantitatively.

Original languageEnglish
Pages (from-to)5243-5248
Number of pages6
JournalLab on a Chip
Volume12
Issue number24
DOIs
Publication statusPublished - 2012 Dec 21
Externally publishedYes

Fingerprint

Stratospheric Ozone
Pulmonary Surfactants
Microfluidics
Phospholipids
Surface-Active Agents
Ozone
Phosphatidylglycerols
Surface active agents
Physical properties
Physics
Lipids
1,2-Dipalmitoylphosphatidylcholine
Bubble formation
Lung
Pulmonary diseases
Oxidative stress
Optical Imaging
Chemical modification
Lung Diseases
Monolayers

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

Cite this

A microfluidic-based bubble generation platform enables analysis of physical property change in phospholipid surfactant layers by interfacial ozone reaction. / Shin, Young Shik; Choi, Tae Su; Kim, Hyungjun; Beauchamp, J. L.; Heath, James R.; Kim, Hugh I.

In: Lab on a Chip, Vol. 12, No. 24, 21.12.2012, p. 5243-5248.

Research output: Contribution to journalArticle

Shin, Young Shik ; Choi, Tae Su ; Kim, Hyungjun ; Beauchamp, J. L. ; Heath, James R. ; Kim, Hugh I. / A microfluidic-based bubble generation platform enables analysis of physical property change in phospholipid surfactant layers by interfacial ozone reaction. In: Lab on a Chip. 2012 ; Vol. 12, No. 24. pp. 5243-5248.
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