A microfluidic detection system based upon a surface immobilized biobarcode assay

Edgar D. Goluch, Savka I. Stoeva, Jae-Seung Lee, Kashan A. Shaikh, Chad A. Mirkin, Chang Liu

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The biobarcode assay (BCA) is capable of achieving low detection limits and high specificity for both protein and DNA targets. The realization of a BCA in a microfluidic format presents unique opportunities and challenges. In this work, we describe a modified form of the BCA called the surface immobilized biobarcode assay (SI-BCA). The SI-BCA employs microchannel walls functionalized with antibodies that bind with the intended targets. Compared with the conventional BCA, it reduces the system complexity and results in shortened process time, which is attributed to significantly reduced diffusion times in the micro-scale channels. Raw serum samples, without any pretreatment, were evaluated with this technique. Prostate specific antigen in the samples was detected at concentrations ranging from 40 pM to 40 fM. The detection limit of the assay using buffer samples is 10 fM. The entire assay, from sample injection to final data analysis was completed in 80 min.

Original languageEnglish
Pages (from-to)2397-2403
Number of pages7
JournalBiosensors and Bioelectronics
Volume24
Issue number8
DOIs
Publication statusPublished - 2009 Apr 15
Externally publishedYes

Fingerprint

Microfluidics
Limit of Detection
Assays
Prostate-Specific Antigen
Buffers
Injections
Antibodies
DNA
Serum
Proteins
Antigens
Microchannels

Keywords

  • Biobarcode assay (BCA)
  • Microfluidic
  • Nanoparticle
  • Prostate specific antigen (PSA)
  • Serum
  • Surface immobilized

ASJC Scopus subject areas

  • Biophysics
  • Biomedical Engineering
  • Biotechnology
  • Electrochemistry

Cite this

A microfluidic detection system based upon a surface immobilized biobarcode assay. / Goluch, Edgar D.; Stoeva, Savka I.; Lee, Jae-Seung; Shaikh, Kashan A.; Mirkin, Chad A.; Liu, Chang.

In: Biosensors and Bioelectronics, Vol. 24, No. 8, 15.04.2009, p. 2397-2403.

Research output: Contribution to journalArticle

Goluch, Edgar D. ; Stoeva, Savka I. ; Lee, Jae-Seung ; Shaikh, Kashan A. ; Mirkin, Chad A. ; Liu, Chang. / A microfluidic detection system based upon a surface immobilized biobarcode assay. In: Biosensors and Bioelectronics. 2009 ; Vol. 24, No. 8. pp. 2397-2403.
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