A microfluidic device for electrofusion of two plant cells

Jongil Ju, Jung Moon Ko, Hyeon Cheol Cha, Sang Hoon Lee

Research output: Contribution to journalConference article

1 Citation (Scopus)

Abstract

In this paper, we have electrofused two cells which are P. japonicum and G. littoralis at the ‘Lab on a Chip’ level. The microchannel platform was fabricated using poly(dimethylsiloxane)(PDMS) via the conventional softlithographic procedure, and the microelectrode patterns (we ebeam evaporated the titanium (as seed layer) and the gold with the thickness of 200 Å and 3000 Å and patterned by the chemical etching) were created on the surface of glass. Both the PDMS platform and electrode glass were aligned and combined via the oxygen plasma-treatment. The channel has two inlets and two types of cells were slowly introduced by using the syringe pumps and we stopped when the cells were positioned around the electrodes. We have applied 1 - 2 MHz AC field (Amplitude: 8 - 10 p-p) with rectangular wave shape to the microelectrodes to form the pearl-chain between the electrodes. Then, the cell-membranes contact closely each other. For the cell fusion, we again applied short DC pulse (amplitude: 250V/mm, duration: 10 – 100 ms) to the microelectrodes. A little pore was generated and finally two cells were fused to single cell. The fused cells were cultured in Nitsch medium containing growth regulators and some cells were cultured. We will investigate their viability with FDA (fluorescein diacetate). This results may encourage the maximum yield of 1: 1 heterologous fusion under the micro environment.

Original languageEnglish
Pages (from-to)302-305
Number of pages4
JournalIFMBE Proceedings
Volume14
Issue number1
Publication statusPublished - 2007
Event10th World Congress on Medical Physics and Biomedical Engineering, WC 2006 - Seoul, Korea, Republic of
Duration: 2006 Aug 272006 Sep 1

Keywords

  • Electrofusion
  • G. littoralis
  • Microfluidic device
  • P. japonicum
  • Poly(dimethylsiloxane)(PDMS)

ASJC Scopus subject areas

  • Bioengineering
  • Biomedical Engineering

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  • Cite this

    Ju, J., Ko, J. M., Cha, H. C., & Lee, S. H. (2007). A microfluidic device for electrofusion of two plant cells. IFMBE Proceedings, 14(1), 302-305.