A microfluidic device for electrofusion of two plant cells

Jongil Ju, Jung Moon Ko, Hyeon Cheol Cha, Sang Hoon Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

In this paper, we have electrofused two cells which are P. japonicum and G. littoralis at the ‘Lab on a Chip’ level. The microchannel platform was fabricated using poly(dimethylsiloxane)(PDMS) via the conventional softlithographic procedure, and the microelectrode patterns (we ebeam evaporated the titanium (as seed layer) and the gold with the thickness of 200 Å and 3000 Å and patterned by the chemical etching) were created on the surface of glass. Both the PDMS platform and electrode glass were aligned and combined via the oxygen plasma-treatment. The channel has two inlets and two types of cells were slowly introduced by using the syringe pumps and we stopped when the cells were positioned around the electrodes. We have applied 1 - 2 MHz AC field (Amplitude: 8 - 10 p-p) with rectangular wave shape to the microelectrodes to form the pearl-chain between the electrodes. Then, the cell-membranes contact closely each other. For the cell fusion, we again applied short DC pulse (amplitude: 250V/mm, duration: 10 – 100 ms) to the microelectrodes. A little pore was generated and finally two cells were fused to single cell. The fused cells were cultured in Nitsch medium containing growth regulators and some cells were cultured. We will investigate their viability with FDA (fluorescein diacetate). This results may encourage the maximum yield of 1: 1 heterologous fusion under the micro environment.

Original languageEnglish
Title of host publicationIFMBE Proceedings
PublisherSpringer Verlag
Pages302-305
Number of pages4
Volume14
Edition1
Publication statusPublished - 2007
Event10th World Congress on Medical Physics and Biomedical Engineering, WC 2006 - Seoul, Korea, Republic of
Duration: 2006 Aug 272006 Sep 1

Other

Other10th World Congress on Medical Physics and Biomedical Engineering, WC 2006
CountryKorea, Republic of
CitySeoul
Period06/8/2706/9/1

Fingerprint

Microelectrodes
Microfluidics
Polydimethylsiloxane
Electrodes
Fusion reactions
Syringes
Glass
Lab-on-a-chip
Cell membranes
Microchannels
Titanium
Gold
Seed
Etching
Pumps
Oxygen
Plasmas
Plant Cells
baysilon

Keywords

  • Electrofusion
  • G. littoralis
  • Microfluidic device
  • P. japonicum
  • Poly(dimethylsiloxane)(PDMS)

ASJC Scopus subject areas

  • Biomedical Engineering
  • Bioengineering

Cite this

Ju, J., Ko, J. M., Cha, H. C., & Lee, S. H. (2007). A microfluidic device for electrofusion of two plant cells. In IFMBE Proceedings (1 ed., Vol. 14, pp. 302-305). Springer Verlag.

A microfluidic device for electrofusion of two plant cells. / Ju, Jongil; Ko, Jung Moon; Cha, Hyeon Cheol; Lee, Sang Hoon.

IFMBE Proceedings. Vol. 14 1. ed. Springer Verlag, 2007. p. 302-305.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Ju, J, Ko, JM, Cha, HC & Lee, SH 2007, A microfluidic device for electrofusion of two plant cells. in IFMBE Proceedings. 1 edn, vol. 14, Springer Verlag, pp. 302-305, 10th World Congress on Medical Physics and Biomedical Engineering, WC 2006, Seoul, Korea, Republic of, 06/8/27.
Ju J, Ko JM, Cha HC, Lee SH. A microfluidic device for electrofusion of two plant cells. In IFMBE Proceedings. 1 ed. Vol. 14. Springer Verlag. 2007. p. 302-305
Ju, Jongil ; Ko, Jung Moon ; Cha, Hyeon Cheol ; Lee, Sang Hoon. / A microfluidic device for electrofusion of two plant cells. IFMBE Proceedings. Vol. 14 1. ed. Springer Verlag, 2007. pp. 302-305
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