A microfluidic flow sensor for measuring cell adhesion

Keon Woo Kwon, Sung Sik Choi, Byungkyu Kim, Se Na Lee, Min Cheol Park, Pilnam Kim, Sang Ho Lee, Seok Ho Park, Kahp Y. Suh

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present a simple, biomarker-free microfluidic device for separating cancer cells from a mixed solution of normal and cancer cells by difference in adhesion force. A polydimethylsiloxane (PDMS) microfluidic chip was fabricated onto glass substrate using standard soft lithography. Three types of polyurethane acrylate (PUA) nanostructure (50nm pillar, 50nm perpendicular groove, 50nm horizontal groove with respect to the direction of flow) were included inside the microfluidic channel by UV-assisted capillary molding. For cell types, MCF7 (breast cancer cell line) and MCF10A (breast normal cell line) were used. To find the optimum condition for separation, each cell line was injected into the microfluidic device and cultured for 1h, 2h, and 3h, respectively, followed by cell detachment by flow of medium solution with increasing flow rate. The adhesion force of MCF10A was stronger than that of MCF7. MCF10A cells cultured onto the nanopatterned surface were more spread than those cultured onto the glass surface. Furthermore, the presence of nanopatterns increased the ratio of adhesion force of normal and cancer cells and thus and the separation efficiency. The optimum culture condition was 2h onto the nanopattern and flow rate was ∼ 300μl/min.

Original languageEnglish
Title of host publication2006 5th IEEE Conference on Sensors
Pages105-108
Number of pages4
DOIs
Publication statusPublished - 2006 Dec 1
Event2006 5th IEEE Conference on Sensors - Daegu, Korea, Republic of
Duration: 2006 Oct 222006 Oct 25

Publication series

NameProceedings of IEEE Sensors

Other

Other2006 5th IEEE Conference on Sensors
CountryKorea, Republic of
CityDaegu
Period06/10/2206/10/25

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ASJC Scopus subject areas

  • Electrical and Electronic Engineering

Cite this

Kwon, K. W., Choi, S. S., Kim, B., Lee, S. N., Park, M. C., Kim, P., Lee, S. H., Park, S. H., & Suh, K. Y. (2006). A microfluidic flow sensor for measuring cell adhesion. In 2006 5th IEEE Conference on Sensors (pp. 105-108). [4178567] (Proceedings of IEEE Sensors). https://doi.org/10.1109/ICSENS.2007.355729