A microfluidic gradient device for drug screening with human iPSC-derived motoneurons

Sung Joon Mo; Ju Hyun Lee; Hyeon Gi Kye; Jong Min Lee; Eun Joong Kim; Dongho Geum; Woong Sun; Bong Geun Chung

doi:10.1039/c9an02384d

A microfluidic gradient device for drug screening with human iPSC-derived motoneurons

Sung Joon Mo, Ju Hyun Lee, Hyeon Gi Kye, Jong Min Lee, Eun Joong Kim, Dongho Geum, Woong Sun, Bong Geun Chung

Department of Biomedical Sciences

Research output: Contribution to journal › Article

Abstract

We developed a microfluidic gradient device to utilize as a drug screening system with human induced pluripotent stem cell (hiPSC)-derived motoneurons. The microfluidic channel was asymmetrically designed to generate the concentration gradients and a micropillar array was used to trap and culture the motoneuron spheroids containing motoneurons for 9 days. We optimized the concentration gradients in the microfluidic device using a computational fluid dynamics (CFD) model. We also observed that the motoneuron spheroid-derived neurite network was generated in response to the concentration gradients of riluzole in the microfluidic device. Therefore, this microfluidic gradient device could be useful for screening of various drugs for neurological disease applications.

Original language	English
Pages (from-to)	3081-3089
Number of pages	9
Journal	Analyst
Volume	145
Issue number	8
DOIs	https://doi.org/10.1039/c9an02384d
Publication status	Published - 2020 Apr 21

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Environmental Chemistry
Spectroscopy
Electrochemistry

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10.1039/c9an02384d

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Mo, S. J., Lee, J. H., Kye, H. G., Lee, J. M., Kim, E. J., Geum, D., Sun, W., & Chung, B. G. (2020). A microfluidic gradient device for drug screening with human iPSC-derived motoneurons. Analyst, 145(8), 3081-3089. https://doi.org/10.1039/c9an02384d

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abstract = "We developed a microfluidic gradient device to utilize as a drug screening system with human induced pluripotent stem cell (hiPSC)-derived motoneurons. The microfluidic channel was asymmetrically designed to generate the concentration gradients and a micropillar array was used to trap and culture the motoneuron spheroids containing motoneurons for 9 days. We optimized the concentration gradients in the microfluidic device using a computational fluid dynamics (CFD) model. We also observed that the motoneuron spheroid-derived neurite network was generated in response to the concentration gradients of riluzole in the microfluidic device. Therefore, this microfluidic gradient device could be useful for screening of various drugs for neurological disease applications.",

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