A microfluidic gradient device for drug screening with human iPSC-derived motoneurons

Sung Joon Mo; Ju Hyun Lee; Hyeon Gi Kye; Jong Min Lee; Eun Joong Kim; Dongho Geum; Woong Sun; Bong Geun Chung

doi:10.1039/c9an02384d

A microfluidic gradient device for drug screening with human iPSC-derived motoneurons

Sung Joon Mo, Ju Hyun Lee, Hyeon Gi Kye, Jong Min Lee, Eun Joong Kim, Dongho Geum, Woong Sun, Bong Geun Chung

Department of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

We developed a microfluidic gradient device to utilize as a drug screening system with human induced pluripotent stem cell (hiPSC)-derived motoneurons. The microfluidic channel was asymmetrically designed to generate the concentration gradients and a micropillar array was used to trap and culture the motoneuron spheroids containing motoneurons for 9 days. We optimized the concentration gradients in the microfluidic device using a computational fluid dynamics (CFD) model. We also observed that the motoneuron spheroid-derived neurite network was generated in response to the concentration gradients of riluzole in the microfluidic device. Therefore, this microfluidic gradient device could be useful for screening of various drugs for neurological disease applications.

Original language	English
Pages (from-to)	3081-3089
Number of pages	9
Journal	Analyst
Volume	145
Issue number	8
DOIs	https://doi.org/10.1039/c9an02384d
Publication status	Published - 2020 Apr 21

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Environmental Chemistry
Spectroscopy
Electrochemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1039/c9an02384d

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title = "A microfluidic gradient device for drug screening with human iPSC-derived motoneurons",

abstract = "We developed a microfluidic gradient device to utilize as a drug screening system with human induced pluripotent stem cell (hiPSC)-derived motoneurons. The microfluidic channel was asymmetrically designed to generate the concentration gradients and a micropillar array was used to trap and culture the motoneuron spheroids containing motoneurons for 9 days. We optimized the concentration gradients in the microfluidic device using a computational fluid dynamics (CFD) model. We also observed that the motoneuron spheroid-derived neurite network was generated in response to the concentration gradients of riluzole in the microfluidic device. Therefore, this microfluidic gradient device could be useful for screening of various drugs for neurological disease applications.",

author = "Mo, {Sung Joon} and Lee, {Ju Hyun} and Kye, {Hyeon Gi} and Lee, {Jong Min} and Kim, {Eun Joong} and Dongho Geum and Woong Sun and Chung, {Bong Geun}",

note = "Funding Information: This work was supported by the National Research Foundation (NRF) of Korea grant funded by the Ministry of Science and ICT (MSIT) (Grant number 2019R1A2C2008863, 2019M3A9H2032547, 2016R1A6A1A03012845). Publisher Copyright: {\textcopyright} 2020 The Royal Society of Chemistry.",

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AU - Lee, Ju Hyun

AU - Kye, Hyeon Gi

AU - Lee, Jong Min

AU - Kim, Eun Joong

AU - Geum, Dongho

AU - Sun, Woong

AU - Chung, Bong Geun

N1 - Funding Information: This work was supported by the National Research Foundation (NRF) of Korea grant funded by the Ministry of Science and ICT (MSIT) (Grant number 2019R1A2C2008863, 2019M3A9H2032547, 2016R1A6A1A03012845). Publisher Copyright: © 2020 The Royal Society of Chemistry.

PY - 2020/4/21

Y1 - 2020/4/21

N2 - We developed a microfluidic gradient device to utilize as a drug screening system with human induced pluripotent stem cell (hiPSC)-derived motoneurons. The microfluidic channel was asymmetrically designed to generate the concentration gradients and a micropillar array was used to trap and culture the motoneuron spheroids containing motoneurons for 9 days. We optimized the concentration gradients in the microfluidic device using a computational fluid dynamics (CFD) model. We also observed that the motoneuron spheroid-derived neurite network was generated in response to the concentration gradients of riluzole in the microfluidic device. Therefore, this microfluidic gradient device could be useful for screening of various drugs for neurological disease applications.

AB - We developed a microfluidic gradient device to utilize as a drug screening system with human induced pluripotent stem cell (hiPSC)-derived motoneurons. The microfluidic channel was asymmetrically designed to generate the concentration gradients and a micropillar array was used to trap and culture the motoneuron spheroids containing motoneurons for 9 days. We optimized the concentration gradients in the microfluidic device using a computational fluid dynamics (CFD) model. We also observed that the motoneuron spheroid-derived neurite network was generated in response to the concentration gradients of riluzole in the microfluidic device. Therefore, this microfluidic gradient device could be useful for screening of various drugs for neurological disease applications.

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A microfluidic gradient device for drug screening with human iPSC-derived motoneurons

Abstract

ASJC Scopus subject areas

UN SDGs

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