A microfluidic multiplexed immunohistochemistry platform for quantitative pathological diagnosis

Minseok S. Kim, Eunsook Lee, Chul Hwan Kim, Chae Yoon Bae, Sun Young Kong, Je Kyun Park

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

A novel microfluidic multiplexed immunohistochemistry (MMIHC) platform was proposed for quantitative pathological diagnosis. Multilayer soft lithography and two-step lithography technologies were used to fabricate MMIHC devices. To overcome the subjective decision of immunohistochemistry (IHC) staining results, image analysis was conducted and quantified with staining intensity and stained area values. By using reversible sealing method between the device and a cellblock sample, not only perfect fluid control for various solutions was exhibited without any leakage, bubble formation and cross-contamination, but also intact sample condition in reaction areas and stable sample preservation were guaranteed. Biomarker examination for estrogen receptor (ER), human epidermal growth factor receptor 2 (HER2), progesterone receptor (PR) and Ki-67 was realized for SK-BR-3 breast cancer cell line at one time. In our experiment, it was revealed that the platform enhanced at least 200-fold reaction efficiency and 24-fold reduction of incubation time. These achievements expect not only savings of time, cost and tissue samples, expanded test for prognostic biomarker candidates and early personalized therapy, but also integration of genomic and proteomic approaches into search for targets in oncology.

Original languageEnglish
Title of host publicationProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages1973-1975
Number of pages3
ISBN (Print)9780979806421
Publication statusPublished - 2009 Jan 1
Event13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of
Duration: 2009 Nov 12009 Nov 5

Other

Other13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
CountryKorea, Republic of
CityJeju
Period09/11/109/11/5

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Keywords

  • Biomarker
  • Immunohistochemistry
  • Multilayer soft lithography
  • Multiplexing
  • Personalized medicine

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Kim, M. S., Lee, E., Kim, C. H., Bae, C. Y., Kong, S. Y., & Park, J. K. (2009). A microfluidic multiplexed immunohistochemistry platform for quantitative pathological diagnosis. In Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1973-1975). Chemical and Biological Microsystems Society.