A microfluidic platform for studying the effects of small temperature gradients in an incubator environment

Sarit K. Das, Seok Chung, Ioannis Zervantonakis, Joseph Atnafu, Roger D. Kamm

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Studies on the effects of variations in temperature and mild temperature gradients on cells, gels, and scaffolds are important from the viewpoint of biological function. Small differences in temperature are known to elicit significant variations in cell behavior and individual protein reactivity. For the study of thermal effects and gradients in vitro, it is important to develop microfluidic platforms which are capable of controlling temperature gradients in an environment which mimics the range of physiological conditions. In the present paper, such a microfluidic thermal gradient system (μTGS) system is proposed which can create and maintain a thermal gradient throughout a cell-seeded gel matrix using the hot and cold water supply integrated in the system in the form of a countercurrent heat exchanger. It is found that a uniform temperature gradient can be created and maintained in the device even inside a high temperature and high humidity environment of an incubator. With the help of a hot and cold circuit controlled from outside the incubator the temperature gradient can be regulated. A numerical simulation of the device demonstrates the thermal feature of the chip. Cell viability and activity under a thermal gradient are examined by placing human breast cancer cells in the device.

Original languageEnglish
Article number034106
JournalBiomicrofluidics
Volume2
Issue number3
DOIs
Publication statusPublished - 2008 Dec 12
Externally publishedYes

Fingerprint

Incubators
Microfluidics
Thermal gradients
temperature gradients
platforms
Hot Temperature
gradients
Temperature
cells
gels
cold water
Equipment and Supplies
heat exchangers
viability
breast
temperature effects
humidity
Gels
reactivity
cancer

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Condensed Matter Physics
  • Materials Science(all)
  • Physical and Theoretical Chemistry

Cite this

A microfluidic platform for studying the effects of small temperature gradients in an incubator environment. / Das, Sarit K.; Chung, Seok; Zervantonakis, Ioannis; Atnafu, Joseph; Kamm, Roger D.

In: Biomicrofluidics, Vol. 2, No. 3, 034106, 12.12.2008.

Research output: Contribution to journalArticle

Das, Sarit K. ; Chung, Seok ; Zervantonakis, Ioannis ; Atnafu, Joseph ; Kamm, Roger D. / A microfluidic platform for studying the effects of small temperature gradients in an incubator environment. In: Biomicrofluidics. 2008 ; Vol. 2, No. 3.
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