A mitochondrial complex I defect impairs cold-regulated nuclear gene expression

Byeong Ha Lee, Hojoung Lee, Liming Xiong, Jian Kang Zhu

Research output: Contribution to journalArticle

166 Citations (Scopus)

Abstract

To study low-temperature signaling in plants, we previously screened for cold stress response mutants using bioluminescent Arabidopsis plants that express the firefly luciferase reporter gene driven by the stress-responsive RD29A promoter. Here, we report on the characterization and cloning of one mutant, frostbite1 (fro1), which shows reduced luminescence induction by cold. fro1 plants display reduced cold induction of stress-responsive genes such as RD29A, KIN1, COR15A, and COR47. fro1 leaves have a reduced capacity for cold acclimation, appear water-soaked, leak electrolytes, and accumulate reactive oxygen species constitutively. FRO1 was isolated through positional cloning and found to encode a protein with high similarity to the 18-kD Fe-S subunit of complex I (NADH dehydrogenase, EC 1.6.5.3) in the mitochondrial electron transfer chain. Confocal imaging shows that the FRO1:green fluorescent protein fusion protein is localized in mitochondria. These results suggest that cold induction of nuclear gene expression is modulated by mitochondrial function.

Original languageEnglish
Pages (from-to)1235-1251
Number of pages17
JournalPlant Cell
Volume14
Issue number6
DOIs
Publication statusPublished - 2002 Jan 1
Externally publishedYes

Fingerprint

NADH dehydrogenase (ubiquinone)
Cloning
Gene expression
Genes
Electron Transport Complex I
Firefly Luciferases
Gene Expression
gene expression
Defects
Mitochondria
Green Fluorescent Proteins
Electrolytes
Luminescence
Organism Cloning
molecular cloning
Reactive Oxygen Species
Proteins
Fusion reactions
Cold-Shock Response
Imaging techniques

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

A mitochondrial complex I defect impairs cold-regulated nuclear gene expression. / Lee, Byeong Ha; Lee, Hojoung; Xiong, Liming; Zhu, Jian Kang.

In: Plant Cell, Vol. 14, No. 6, 01.01.2002, p. 1235-1251.

Research output: Contribution to journalArticle

Lee, Byeong Ha ; Lee, Hojoung ; Xiong, Liming ; Zhu, Jian Kang. / A mitochondrial complex I defect impairs cold-regulated nuclear gene expression. In: Plant Cell. 2002 ; Vol. 14, No. 6. pp. 1235-1251.
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