Abstract
To study low-temperature signaling in plants, we previously screened for cold stress response mutants using bioluminescent Arabidopsis plants that express the firefly luciferase reporter gene driven by the stress-responsive RD29A promoter. Here, we report on the characterization and cloning of one mutant, frostbite1 (fro1), which shows reduced luminescence induction by cold. fro1 plants display reduced cold induction of stress-responsive genes such as RD29A, KIN1, COR15A, and COR47. fro1 leaves have a reduced capacity for cold acclimation, appear water-soaked, leak electrolytes, and accumulate reactive oxygen species constitutively. FRO1 was isolated through positional cloning and found to encode a protein with high similarity to the 18-kD Fe-S subunit of complex I (NADH dehydrogenase, EC 1.6.5.3) in the mitochondrial electron transfer chain. Confocal imaging shows that the FRO1:green fluorescent protein fusion protein is localized in mitochondria. These results suggest that cold induction of nuclear gene expression is modulated by mitochondrial function.
| Original language | English |
|---|---|
| Pages (from-to) | 1235-1251 |
| Number of pages | 17 |
| Journal | Plant Cell |
| Volume | 14 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2002 Jan 1 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Plant Science
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Cell Biology
Cite this
A mitochondrial complex I defect impairs cold-regulated nuclear gene expression. / Lee, Byeong Ha; Lee, Hojoung; Xiong, Liming; Zhu, Jian Kang.
In: Plant Cell, Vol. 14, No. 6, 01.01.2002, p. 1235-1251.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A mitochondrial complex I defect impairs cold-regulated nuclear gene expression
AU - Lee, Byeong Ha
AU - Lee, Hojoung
AU - Xiong, Liming
AU - Zhu, Jian Kang
PY - 2002/1/1
Y1 - 2002/1/1
N2 - To study low-temperature signaling in plants, we previously screened for cold stress response mutants using bioluminescent Arabidopsis plants that express the firefly luciferase reporter gene driven by the stress-responsive RD29A promoter. Here, we report on the characterization and cloning of one mutant, frostbite1 (fro1), which shows reduced luminescence induction by cold. fro1 plants display reduced cold induction of stress-responsive genes such as RD29A, KIN1, COR15A, and COR47. fro1 leaves have a reduced capacity for cold acclimation, appear water-soaked, leak electrolytes, and accumulate reactive oxygen species constitutively. FRO1 was isolated through positional cloning and found to encode a protein with high similarity to the 18-kD Fe-S subunit of complex I (NADH dehydrogenase, EC 1.6.5.3) in the mitochondrial electron transfer chain. Confocal imaging shows that the FRO1:green fluorescent protein fusion protein is localized in mitochondria. These results suggest that cold induction of nuclear gene expression is modulated by mitochondrial function.
AB - To study low-temperature signaling in plants, we previously screened for cold stress response mutants using bioluminescent Arabidopsis plants that express the firefly luciferase reporter gene driven by the stress-responsive RD29A promoter. Here, we report on the characterization and cloning of one mutant, frostbite1 (fro1), which shows reduced luminescence induction by cold. fro1 plants display reduced cold induction of stress-responsive genes such as RD29A, KIN1, COR15A, and COR47. fro1 leaves have a reduced capacity for cold acclimation, appear water-soaked, leak electrolytes, and accumulate reactive oxygen species constitutively. FRO1 was isolated through positional cloning and found to encode a protein with high similarity to the 18-kD Fe-S subunit of complex I (NADH dehydrogenase, EC 1.6.5.3) in the mitochondrial electron transfer chain. Confocal imaging shows that the FRO1:green fluorescent protein fusion protein is localized in mitochondria. These results suggest that cold induction of nuclear gene expression is modulated by mitochondrial function.
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U2 - 10.1105/tpc.010433
DO - 10.1105/tpc.010433
M3 - Article
C2 - 12084824
AN - SCOPUS:0035983840
VL - 14
SP - 1235
EP - 1251
JO - Plant Cell
JF - Plant Cell
SN - 1040-4651
IS - 6
ER -