A mitochondrial-targeted two-photon probe for zinc ion

Goutam Masanta, Chang Su Lim, Hyung Joong Kim, Ji Hee Han, Hwan Myung Kim, Bong-Rae Cho

Research output: Contribution to journalArticle

185 Citations (Scopus)

Abstract

We report a two-photon probe (SZn-Mito) for mitochondrial zinc ions ([Zn2+]m). This probe shows a 7-fold enhancement of two-photon-excited fluorescence in response to Zn2+ with a dissociation constant (KdTP) of 3.1 ± 0.1 nM and pH insensitivity in the biologically relevant range, allowing the detection of [Zn2+] m in a rat hippocampal slice at a depth of 100-200 μm without interference from other metal ions through the use of two-photon microscopy.

Original languageEnglish
Pages (from-to)5698-5700
Number of pages3
JournalJournal of the American Chemical Society
Volume133
Issue number15
DOIs
Publication statusPublished - 2011 Apr 20

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Photons
Zinc
Ions
Metal ions
Rats
Microscopy
Microscopic examination
Fluorescence
Metals

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

A mitochondrial-targeted two-photon probe for zinc ion. / Masanta, Goutam; Lim, Chang Su; Kim, Hyung Joong; Han, Ji Hee; Kim, Hwan Myung; Cho, Bong-Rae.

In: Journal of the American Chemical Society, Vol. 133, No. 15, 20.04.2011, p. 5698-5700.

Research output: Contribution to journalArticle

Masanta, G, Lim, CS, Kim, HJ, Han, JH, Kim, HM & Cho, B-R 2011, 'A mitochondrial-targeted two-photon probe for zinc ion', Journal of the American Chemical Society, vol. 133, no. 15, pp. 5698-5700. https://doi.org/10.1021/ja200444t
Masanta, Goutam ; Lim, Chang Su ; Kim, Hyung Joong ; Han, Ji Hee ; Kim, Hwan Myung ; Cho, Bong-Rae. / A mitochondrial-targeted two-photon probe for zinc ion. In: Journal of the American Chemical Society. 2011 ; Vol. 133, No. 15. pp. 5698-5700.
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