A mitochondrial-targeted two-photon probe for zinc ion

Goutam Masanta, Chang Su Lim, Hyung Joong Kim, Ji Hee Han, Hwan Myung Kim, Bong Rae Cho

Research output: Contribution to journalArticle

191 Citations (Scopus)

Abstract

We report a two-photon probe (SZn-Mito) for mitochondrial zinc ions ([Zn2+]m). This probe shows a 7-fold enhancement of two-photon-excited fluorescence in response to Zn2+ with a dissociation constant (KdTP) of 3.1 ± 0.1 nM and pH insensitivity in the biologically relevant range, allowing the detection of [Zn2+] m in a rat hippocampal slice at a depth of 100-200 μm without interference from other metal ions through the use of two-photon microscopy.

Original languageEnglish
Pages (from-to)5698-5700
Number of pages3
JournalJournal of the American Chemical Society
Volume133
Issue number15
DOIs
Publication statusPublished - 2011 Apr 20

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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