A monitoring system for axonal growth dynamics using micropatterns of permissive and Semaphorin 3F chemorepulsive signals

Jae Ryun Ryu, June Hoan Kim, Hyo Min Cho, Youhwa Jo, Boram Lee, Sunghoon Joo, Uikyu Chae, Yoonkey Nam, Il Joo Cho, Woong Sun

Research output: Contribution to journalArticle

Abstract

Neurons reach their correct targets by directional outgrowth of axons, which is mediated by attractive or repulsive cues. Growing axons occasionally cross a field of repulsive cues and stop at intermediate targets on the journey to their final destination. However, it is not well-understood how individual growth cones make decisions, and pass through repulsive territory to reach their permissive target regions. We developed a microcontact printing culture system that could trap individual axonal tips in a permissive dot area surrounded by the repulsive signal, semaphorin 3F (Sema3F). Axons of rat hippocampal neurons on the Sema3F/PLL dot array extended in the checkboard pattern with a significantly slow growth rate. The detailed analysis of the behaviors of axonal growth cones revealed the saccadic dynamics in the dot array system. The trapped axonal tips in the permissive area underwent growth cone enlargement with remarkably spiky filopodia, promoting their escape from the Sema3F constraints with straight extension of axons. This structured axonal growth on the dot pattern was disrupted by increased inter-dot distance, or perturbing intracellular signaling machineries. These data indicate that axons grow against repulsive signals by jumping over the repulsive cues, depending on contact signals and intracellular milieu. Our study suggests that our dot array culture system can be used as a screening system to easily and efficiently evaluate ECM or small molecule inhibitors interfering growth cone dynamics leading to controlling axonal growth.

Original languageEnglish
Pages (from-to)291-305
Number of pages15
JournalLab on a chip
Volume19
Issue number2
DOIs
Publication statusPublished - 2019 Jan 1

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Semaphorins
Growth Cones
Axons
Cues
Cones
Monitoring
Growth
Neurons
Printing
Pseudopodia
Military electronic countermeasures
Phase locked loops
Rats
Screening
Molecules

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

Cite this

A monitoring system for axonal growth dynamics using micropatterns of permissive and Semaphorin 3F chemorepulsive signals. / Ryu, Jae Ryun; Kim, June Hoan; Cho, Hyo Min; Jo, Youhwa; Lee, Boram; Joo, Sunghoon; Chae, Uikyu; Nam, Yoonkey; Cho, Il Joo; Sun, Woong.

In: Lab on a chip, Vol. 19, No. 2, 01.01.2019, p. 291-305.

Research output: Contribution to journalArticle

Ryu, Jae Ryun ; Kim, June Hoan ; Cho, Hyo Min ; Jo, Youhwa ; Lee, Boram ; Joo, Sunghoon ; Chae, Uikyu ; Nam, Yoonkey ; Cho, Il Joo ; Sun, Woong. / A monitoring system for axonal growth dynamics using micropatterns of permissive and Semaphorin 3F chemorepulsive signals. In: Lab on a chip. 2019 ; Vol. 19, No. 2. pp. 291-305.
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