A multichannel neural probe with embedded microfluidic channels for simultaneous in vivo neural recording and drug delivery

Hyunjoo J. Lee, Yoojin Son, Jeongyeon Kim, Changjoon Lee, Eui Sung Yoon, Il Joo Cho

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Multi-functional neural probes integrated with various stimulation modalities are becoming essential tools in neuroscience to study the brain more effectively. In this paper, we present a new multi-functional neural probe that allows chemical stimulation through drug delivery and simultaneous recording of individual neuron signals through a microelectrode array. By embedding microchannels in silicon using a proposed glass reflow process, we successfully fabricated 40 μm thick silicon neural probes suitable for small animal experiments. The electrochemical impedance spectroscopy confirms that impedance of iridium microelectrodes is low enough (<1 MΩ at 1 kHz) to measure neural signals. Flow rate characterization in a 0.9% w/v agarose gel shows the capability to deliver a small volume of drugs (<1 μl) at a controlled flow rate. We demonstrate the viability and potential of this new probe by conducting in vivo experiments on mice. Because of the proposed compact structure, both action potentials of individual neurons and local field potentials (LFP) at the thalamus region of a mouse brain were successfully detected with a noise level of ~30 μVpp. Furthermore, we successfully induced absence seizure by injecting seizure-inducing drugs (baclofen) at a local target region and observed distinctive changes in neural signal patterns. Specifically, spike-wave discharge (SWD), which is an indicative signal pattern of absence seizure, was successfully recorded. These signals were also directly compared to SWD detected after inducing absence seizure through direct injection of baclofen through the abdomen. This work demonstrates the potential of our multi-functional neural probes for use in effective investigation of brain functions and disorders by using widely available mouse models.

Original languageEnglish
Pages (from-to)1590-1597
Number of pages8
JournalLab on a Chip - Miniaturisation for Chemistry and Biology
Volume15
Issue number6
DOIs
Publication statusPublished - 2015
Externally publishedYes

Fingerprint

Absence Epilepsy
Microfluidics
Drug delivery
Baclofen
Microelectrodes
Silicon
Brain
Pharmaceutical Preparations
Chemical Stimulation
Dielectric Spectroscopy
Neurons
Iridium
Brain Diseases
Neurosciences
Thalamus
Electric Impedance
Abdomen
Sepharose
Action Potentials
Glass

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)
  • Bioengineering
  • Biomedical Engineering

Cite this

A multichannel neural probe with embedded microfluidic channels for simultaneous in vivo neural recording and drug delivery. / Lee, Hyunjoo J.; Son, Yoojin; Kim, Jeongyeon; Lee, Changjoon; Yoon, Eui Sung; Cho, Il Joo.

In: Lab on a Chip - Miniaturisation for Chemistry and Biology, Vol. 15, No. 6, 2015, p. 1590-1597.

Research output: Contribution to journalArticle

Lee, Hyunjoo J. ; Son, Yoojin ; Kim, Jeongyeon ; Lee, Changjoon ; Yoon, Eui Sung ; Cho, Il Joo. / A multichannel neural probe with embedded microfluidic channels for simultaneous in vivo neural recording and drug delivery. In: Lab on a Chip - Miniaturisation for Chemistry and Biology. 2015 ; Vol. 15, No. 6. pp. 1590-1597.
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