A myristoylated pseudosubstrate peptide of PKC-ζ induces degranulation in HMC-1 cells independently of PKC-ζ activity

Seyoung Lim, Jung Woong Choi, Hyeon Soo Kim, Yun Hee Kim, Kyungmoo Yea, Kyun Heo, Jong Hyun Kim, Sun Hee Kim, Minseok Song, Jae Il Kim, Sung Ho Ryu, Pann Ghill Suh

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Mast cells play a central role in allergic disease and host defense against several pathogens through the release of various bioactive compounds via degranulation. In this study, we found that a myristoylated pseudosubstrate of PKC-ζ (ζ-PS; myristoyl-SIYRRGARRWRKL, a PKC-ζ inhibitor) regulates mast cell degranulation. ζ-PS increased [Ca+2]i level at nanomolar concentrations in a PKC-ζ activity-independent manner in HMC-1 cells. Moreover, ζ-PS-induced [Ca+2]i generation was completely abrogated by phospholipase C (PLC), IP3 receptor or Gαi/o inhibitor and ζ-PS potently induced degranulation in HMC-1 cells which was significantly inhibited by pretreating PLC inhibitors or a calcium chelator. Therefore, our results suggest that ζ-PS can induce degranulation in HMC-1 cells by triggering the calcium signal via a PKC-ζ-independent but Gαi/o, PLC and IP3-dependent pathways.

Original languageEnglish
Pages (from-to)733-740
Number of pages8
JournalLife Sciences
Volume82
Issue number13-14
DOIs
Publication statusPublished - 2008 Mar 26
Externally publishedYes

Fingerprint

Type C Phospholipases
Mast Cells
Peptides
Inositol 1,4,5-Trisphosphate Receptors
Cell Degranulation
Pathogens
Calcium

ASJC Scopus subject areas

  • Pharmacology

Cite this

A myristoylated pseudosubstrate peptide of PKC-ζ induces degranulation in HMC-1 cells independently of PKC-ζ activity. / Lim, Seyoung; Choi, Jung Woong; Kim, Hyeon Soo; Kim, Yun Hee; Yea, Kyungmoo; Heo, Kyun; Kim, Jong Hyun; Kim, Sun Hee; Song, Minseok; Kim, Jae Il; Ryu, Sung Ho; Suh, Pann Ghill.

In: Life Sciences, Vol. 82, No. 13-14, 26.03.2008, p. 733-740.

Research output: Contribution to journalArticle

Lim, S, Choi, JW, Kim, HS, Kim, YH, Yea, K, Heo, K, Kim, JH, Kim, SH, Song, M, Kim, JI, Ryu, SH & Suh, PG 2008, 'A myristoylated pseudosubstrate peptide of PKC-ζ induces degranulation in HMC-1 cells independently of PKC-ζ activity', Life Sciences, vol. 82, no. 13-14, pp. 733-740. https://doi.org/10.1016/j.lfs.2008.01.005
Lim, Seyoung ; Choi, Jung Woong ; Kim, Hyeon Soo ; Kim, Yun Hee ; Yea, Kyungmoo ; Heo, Kyun ; Kim, Jong Hyun ; Kim, Sun Hee ; Song, Minseok ; Kim, Jae Il ; Ryu, Sung Ho ; Suh, Pann Ghill. / A myristoylated pseudosubstrate peptide of PKC-ζ induces degranulation in HMC-1 cells independently of PKC-ζ activity. In: Life Sciences. 2008 ; Vol. 82, No. 13-14. pp. 733-740.
@article{e61bf3a766504c01b3a84fb742bd9e82,
title = "A myristoylated pseudosubstrate peptide of PKC-ζ induces degranulation in HMC-1 cells independently of PKC-ζ activity",
abstract = "Mast cells play a central role in allergic disease and host defense against several pathogens through the release of various bioactive compounds via degranulation. In this study, we found that a myristoylated pseudosubstrate of PKC-ζ (ζ-PS; myristoyl-SIYRRGARRWRKL, a PKC-ζ inhibitor) regulates mast cell degranulation. ζ-PS increased [Ca+2]i level at nanomolar concentrations in a PKC-ζ activity-independent manner in HMC-1 cells. Moreover, ζ-PS-induced [Ca+2]i generation was completely abrogated by phospholipase C (PLC), IP3 receptor or Gαi/o inhibitor and ζ-PS potently induced degranulation in HMC-1 cells which was significantly inhibited by pretreating PLC inhibitors or a calcium chelator. Therefore, our results suggest that ζ-PS can induce degranulation in HMC-1 cells by triggering the calcium signal via a PKC-ζ-independent but Gαi/o, PLC and IP3-dependent pathways.",
keywords = "ζ-PS, Calcium, Degranulation, IP, Mast cells, Phospholipase C",
author = "Seyoung Lim and Choi, {Jung Woong} and Kim, {Hyeon Soo} and Kim, {Yun Hee} and Kyungmoo Yea and Kyun Heo and Kim, {Jong Hyun} and Kim, {Sun Hee} and Minseok Song and Kim, {Jae Il} and Ryu, {Sung Ho} and Suh, {Pann Ghill}",
year = "2008",
month = "3",
day = "26",
doi = "10.1016/j.lfs.2008.01.005",
language = "English",
volume = "82",
pages = "733--740",
journal = "Life Sciences",
issn = "0024-3205",
publisher = "Elsevier Inc.",
number = "13-14",

}

TY - JOUR

T1 - A myristoylated pseudosubstrate peptide of PKC-ζ induces degranulation in HMC-1 cells independently of PKC-ζ activity

AU - Lim, Seyoung

AU - Choi, Jung Woong

AU - Kim, Hyeon Soo

AU - Kim, Yun Hee

AU - Yea, Kyungmoo

AU - Heo, Kyun

AU - Kim, Jong Hyun

AU - Kim, Sun Hee

AU - Song, Minseok

AU - Kim, Jae Il

AU - Ryu, Sung Ho

AU - Suh, Pann Ghill

PY - 2008/3/26

Y1 - 2008/3/26

N2 - Mast cells play a central role in allergic disease and host defense against several pathogens through the release of various bioactive compounds via degranulation. In this study, we found that a myristoylated pseudosubstrate of PKC-ζ (ζ-PS; myristoyl-SIYRRGARRWRKL, a PKC-ζ inhibitor) regulates mast cell degranulation. ζ-PS increased [Ca+2]i level at nanomolar concentrations in a PKC-ζ activity-independent manner in HMC-1 cells. Moreover, ζ-PS-induced [Ca+2]i generation was completely abrogated by phospholipase C (PLC), IP3 receptor or Gαi/o inhibitor and ζ-PS potently induced degranulation in HMC-1 cells which was significantly inhibited by pretreating PLC inhibitors or a calcium chelator. Therefore, our results suggest that ζ-PS can induce degranulation in HMC-1 cells by triggering the calcium signal via a PKC-ζ-independent but Gαi/o, PLC and IP3-dependent pathways.

AB - Mast cells play a central role in allergic disease and host defense against several pathogens through the release of various bioactive compounds via degranulation. In this study, we found that a myristoylated pseudosubstrate of PKC-ζ (ζ-PS; myristoyl-SIYRRGARRWRKL, a PKC-ζ inhibitor) regulates mast cell degranulation. ζ-PS increased [Ca+2]i level at nanomolar concentrations in a PKC-ζ activity-independent manner in HMC-1 cells. Moreover, ζ-PS-induced [Ca+2]i generation was completely abrogated by phospholipase C (PLC), IP3 receptor or Gαi/o inhibitor and ζ-PS potently induced degranulation in HMC-1 cells which was significantly inhibited by pretreating PLC inhibitors or a calcium chelator. Therefore, our results suggest that ζ-PS can induce degranulation in HMC-1 cells by triggering the calcium signal via a PKC-ζ-independent but Gαi/o, PLC and IP3-dependent pathways.

KW - ζ-PS

KW - Calcium

KW - Degranulation

KW - IP

KW - Mast cells

KW - Phospholipase C

UR - http://www.scopus.com/inward/record.url?scp=40849094196&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=40849094196&partnerID=8YFLogxK

U2 - 10.1016/j.lfs.2008.01.005

DO - 10.1016/j.lfs.2008.01.005

M3 - Article

VL - 82

SP - 733

EP - 740

JO - Life Sciences

JF - Life Sciences

SN - 0024-3205

IS - 13-14

ER -