TY - JOUR
T1 - A new coccolith modified electrode-based biosensor using a cognate pair of aptamers with sandwich-type binding
AU - Kim, Sang Hoon
AU - Nam, Onyou
AU - Jin, Eon Seon
AU - Gu, Man Bock
N1 - Funding Information:
This work was supported by the Basic Core Technology Development Program for the Oceans and the Polar Regions of the National Research Foundation of Korea(NRF) funded by the Ministry of Science, ICT ( 2015M1A5A1037055 ), and by the National Research Foundation of Korea (NRF) grant funded by the Korea Government (MSIP) (No. 2016R1A2B3011422 ), and by a Korea University Grant . Also, we would like to thank Mr. Cheulmin Joe for his help in preparing the coccolith modified electrodeposited electrode.
Publisher Copyright:
© 2018 Elsevier B.V.
PY - 2019/1/1
Y1 - 2019/1/1
N2 - In this study, we report a cognate pair of the aptamer-based sandwich-type electrochemical biosensor for type 2 diabetes biomarker (Vaspin) using coccolith modified electrodeposited on the screen-printed gold electrode (CME-SPGE). The coccolith derived from E. huxleyi used in this study were known to be highly-structured microparticles with many nano-sized pores. The CME-SPGE was successfully fabricated by drop-casting coccoliths, followed by Au sputtering and electrodeposition of Au. On this CME-SPGE electrode, the sandwich-type electrochemical aptasensor was fabricated by using a cognate pair of aptamers. The morphological, electrochemical characteristics and the performances of both the CME-SPGE and the completely fabricated sandwich-type aptasensor were investigated by SEM, EDAX, cyclic voltammetry, and chronoamperometry. Due to the synergic effect of a cognate pair of aptamers on CME-SPGE, this newly developed sandwich-type electrochemical biosensor for Vaspin showed high specificity, and good sensitivity with a limit of detection (LOD) of 298 pM, along with more widen the linear range. To the best of our knowledge, this is the first report about the use of a coccolith modified electrode with a cognate pair aptamer resulting in sandwich-type binding in an electrochemical biosensor. With the advantages of using highly-structured biomineral microparticles and a cognate pair of aptamers, this new study may pave the innovative way to design a novel sandwich-type electrochemical aptasensor platform.
AB - In this study, we report a cognate pair of the aptamer-based sandwich-type electrochemical biosensor for type 2 diabetes biomarker (Vaspin) using coccolith modified electrodeposited on the screen-printed gold electrode (CME-SPGE). The coccolith derived from E. huxleyi used in this study were known to be highly-structured microparticles with many nano-sized pores. The CME-SPGE was successfully fabricated by drop-casting coccoliths, followed by Au sputtering and electrodeposition of Au. On this CME-SPGE electrode, the sandwich-type electrochemical aptasensor was fabricated by using a cognate pair of aptamers. The morphological, electrochemical characteristics and the performances of both the CME-SPGE and the completely fabricated sandwich-type aptasensor were investigated by SEM, EDAX, cyclic voltammetry, and chronoamperometry. Due to the synergic effect of a cognate pair of aptamers on CME-SPGE, this newly developed sandwich-type electrochemical biosensor for Vaspin showed high specificity, and good sensitivity with a limit of detection (LOD) of 298 pM, along with more widen the linear range. To the best of our knowledge, this is the first report about the use of a coccolith modified electrode with a cognate pair aptamer resulting in sandwich-type binding in an electrochemical biosensor. With the advantages of using highly-structured biomineral microparticles and a cognate pair of aptamers, this new study may pave the innovative way to design a novel sandwich-type electrochemical aptasensor platform.
KW - A cognate pair of aptamers
KW - Chronoamperometry
KW - Coccolith
KW - Electrochemical biosensor
KW - Sandwich-type binding
KW - Type 2 diabetes
UR - http://www.scopus.com/inward/record.url?scp=85051699405&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2018.08.021
DO - 10.1016/j.bios.2018.08.021
M3 - Article
C2 - 30139622
AN - SCOPUS:85051699405
SN - 0956-5663
VL - 123
SP - 160
EP - 166
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
ER -
