Abstract
A new lateral flow strip assay (LFSA) using a pair of aptamers has been designed and successfully developed using a pair of aptamers-functionalized with gold nanoparticles (AuNPs). This new LFSA biosensor system utilizes a cognate aptamer duo binding to vaspin, a target protein, at the two different binding sites, and exhibited a sensitive and highly selective response to vaspin. A sandwich-type format in LFSA was developed based on biotin-labeled primary V1 aptamer immobilized on streptavidin coated membrane as a capturing probe and secondary V49 aptamer conjugated with AuNPs as a signaling probe. Using this LFSA, vaspin could be visibly observed within the detectable concentrations of vaspin up to 5. nM in both buffer and serum conditions. The sensitivity of this LFSA developed in this study was ranged from 0.137 to 25. nM in buffer and from 0.105 to 25. nM in spiked human serum, respectively. The limit of detection (LOD) of this LFSA was found to be 0.137. nM and 0.105. nM in buffer and spiked human serum condition, respectively. Therefore, this study has shown successful development of a simple yet effective LFSA for vaspin detection, and this development is not only limited to this target, but also other targets with a pair of aptamers available, without any special and laborious instrumentations. This system will be particularly useful as a screening tool for rapid on-site detection of any targets with a pair of aptamers generated.
| Original language | English |
|---|---|
| Journal | Biosensors and Bioelectronics |
| DOIs | |
| Publication status | Accepted/In press - 2016 Jun 16 |
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Keywords
- Cognate aptamer duo
- Lateral flow strip assay (LFSA)
- Sandwich-type
- Vaspin
ASJC Scopus subject areas
- Biotechnology
- Biophysics
- Biomedical Engineering
- Electrochemistry
Cite this
A new lateral flow strip assay (LFSA) using a pair of aptamers for the detection of Vaspin. / Ahmad Raston, Nurul Hanun; Nguyen, Van Thuan; Gu, Man Bock.
In: Biosensors and Bioelectronics, 16.06.2016.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A new lateral flow strip assay (LFSA) using a pair of aptamers for the detection of Vaspin
AU - Ahmad Raston, Nurul Hanun
AU - Nguyen, Van Thuan
AU - Gu, Man Bock
PY - 2016/6/16
Y1 - 2016/6/16
N2 - A new lateral flow strip assay (LFSA) using a pair of aptamers has been designed and successfully developed using a pair of aptamers-functionalized with gold nanoparticles (AuNPs). This new LFSA biosensor system utilizes a cognate aptamer duo binding to vaspin, a target protein, at the two different binding sites, and exhibited a sensitive and highly selective response to vaspin. A sandwich-type format in LFSA was developed based on biotin-labeled primary V1 aptamer immobilized on streptavidin coated membrane as a capturing probe and secondary V49 aptamer conjugated with AuNPs as a signaling probe. Using this LFSA, vaspin could be visibly observed within the detectable concentrations of vaspin up to 5. nM in both buffer and serum conditions. The sensitivity of this LFSA developed in this study was ranged from 0.137 to 25. nM in buffer and from 0.105 to 25. nM in spiked human serum, respectively. The limit of detection (LOD) of this LFSA was found to be 0.137. nM and 0.105. nM in buffer and spiked human serum condition, respectively. Therefore, this study has shown successful development of a simple yet effective LFSA for vaspin detection, and this development is not only limited to this target, but also other targets with a pair of aptamers available, without any special and laborious instrumentations. This system will be particularly useful as a screening tool for rapid on-site detection of any targets with a pair of aptamers generated.
AB - A new lateral flow strip assay (LFSA) using a pair of aptamers has been designed and successfully developed using a pair of aptamers-functionalized with gold nanoparticles (AuNPs). This new LFSA biosensor system utilizes a cognate aptamer duo binding to vaspin, a target protein, at the two different binding sites, and exhibited a sensitive and highly selective response to vaspin. A sandwich-type format in LFSA was developed based on biotin-labeled primary V1 aptamer immobilized on streptavidin coated membrane as a capturing probe and secondary V49 aptamer conjugated with AuNPs as a signaling probe. Using this LFSA, vaspin could be visibly observed within the detectable concentrations of vaspin up to 5. nM in both buffer and serum conditions. The sensitivity of this LFSA developed in this study was ranged from 0.137 to 25. nM in buffer and from 0.105 to 25. nM in spiked human serum, respectively. The limit of detection (LOD) of this LFSA was found to be 0.137. nM and 0.105. nM in buffer and spiked human serum condition, respectively. Therefore, this study has shown successful development of a simple yet effective LFSA for vaspin detection, and this development is not only limited to this target, but also other targets with a pair of aptamers available, without any special and laborious instrumentations. This system will be particularly useful as a screening tool for rapid on-site detection of any targets with a pair of aptamers generated.
KW - Cognate aptamer duo
KW - Lateral flow strip assay (LFSA)
KW - Sandwich-type
KW - Vaspin
UR - http://www.scopus.com/inward/record.url?scp=85007507149&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85007507149&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2016.11.061
DO - 10.1016/j.bios.2016.11.061
M3 - Article
JO - Biosensors
JF - Biosensors
SN - 0956-5663
ER -