A new shuttle plasmid that stably replicates in clostridium acetobutylicum

Sang Hyun Lee, Min A. Kwon, Sunhwa Choi, Sooah Kim, Jungyeon Kim, Yong An Shin, Kyoung Heon Kim

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We have developed a new shuttle plasmid, designated as pLK1-MCS that can replicate in both Clostridium acetobutylicum and Escherichia coli, by combining the pUB110 and pUC19 plasmids. Plasmid pLK1-MCS replicated more stably than previously reported plasmids containing either the pIM13 or the pAMβ1 replicon in the absence of antibiotic selective pressure. The transfer frequency of pLK1-MCS into C. acetobutylicum was similar to the transfer frequency of other shuttle plasmids. We complemented C. acetobutylicum ML1 (that does not produce solvents such as acetone, butanol, and ethanol owing to loss of the megaplasmid pSOL1 harboring the adhE1-ctfAB-adc operon) by introducing pLK1-MCS carrying the adhE1-ctfAB-adc operon into C. acetobutylicum ML1. The transformed cells were able to resume anaerobic solvent production, indicating that the new shuttle plasmid has the potential for practical use in microbial biotechnology.

Original languageEnglish
Article numberA017
Pages (from-to)1702-1708
Number of pages7
JournalJournal of Microbiology and Biotechnology
Volume25
Issue number10
DOIs
Publication statusPublished - 2015

Keywords

  • Clostridium acetobutylicum
  • Segregational stability
  • Shuttle plasmid

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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