A new spin filter for high density culture and ethanol production by Saccharomyces cerevisiae

Hyunsoo Moon, Dong Joon Lim, Gu Young Song, Hyun Ah Kang, Seung Wook Kim, Ik Hwan Kim, Suk In Hong

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A new spin filter consisting of 50 μm (nominal pore size) depth filters rolled on a stainless steel grid was developed, using Saccharomyces cerevisiae as a model suspension cell to evaluate the spin filter performance. In a 1.8-1 fermentor with a rotation speed of 300 rpm and perfusion rate of 4 ml/min, a cell concentration of 49 g/l and ethanol concentration of 45 g/l from 100 g/l glucose could be obtained in a perfusion culture. The major mechanisms for cell separation used by the large-pore spin filter appeared to be centrifugal force and pivotal movement of the cells in the spin filter.

Original languageEnglish
Pages (from-to)406-410
Number of pages5
JournalJournal of microbiology and biotechnology
Volume12
Issue number3
Publication statusPublished - 2002

Keywords

  • Centrifugal force
  • High density culture
  • Pivotal movement
  • Spin filter
  • Yeast

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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