A novel alkaline lipase from Ralstonia with potential application in biodiesel production

Hah Young Yoo; Jaya Ram Simkhada; Seung Sik Cho; Don Hee Park; Seung Wook Kim; Chi Nam Seong; Jin Cheol Yoo

doi:10.1016/j.biortech.2011.02.046

A novel alkaline lipase from Ralstonia with potential application in biodiesel production

Hah Young Yoo, Jaya Ram Simkhada, Seung Sik Cho, Don Hee Park, Seung Wook Kim, Chi Nam Seong, Jin Cheol Yoo

Department of Chemical and Biological Engineering

Research output: Contribution to journal › Article › peer-review

62 Citations (Scopus)

Abstract

With the aim of isolating a biocatalyst able to catalyze biodiesel production from microbial source, Ralstonia sp. CS274 was isolated and a lipase from the strain (RL74) was purified. Molecular weight of RL74 was estimated to be 28,000Da by SDS-PAGE. The activity was highest at 50-55°C and pH 8.0-9.5 and was stable at pH 7.0-12.0 and up to 45°C. It was resistant to oxidizing and reducing agents and the activity was enhanced by detergents. RL74 was 1,3 specific and K_m and V_max for p-nitrophenyl palmitate were 2.73±0.6mM and 101.4±1.9mM/minmg, respectively. N-terminal amino acid sequence showed partial homology with that of Penicillium lipases. RL74 produced biodiesel more efficiently in palm oil than in soybean oil; and the production was highest at pH 8.0, at 5% methanol and at 20% water content.

Original language	English
Pages (from-to)	6104-6111
Number of pages	8
Journal	Bioresource technology
Volume	102
Issue number	10
DOIs	https://doi.org/10.1016/j.biortech.2011.02.046
Publication status	Published - 2011 May

Keywords

Biodiesel production
Lipase
Purification
Ralstonia sp. CS274

ASJC Scopus subject areas

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biortech.2011.02.046

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title = "A novel alkaline lipase from Ralstonia with potential application in biodiesel production",

abstract = "With the aim of isolating a biocatalyst able to catalyze biodiesel production from microbial source, Ralstonia sp. CS274 was isolated and a lipase from the strain (RL74) was purified. Molecular weight of RL74 was estimated to be 28,000Da by SDS-PAGE. The activity was highest at 50-55°C and pH 8.0-9.5 and was stable at pH 7.0-12.0 and up to 45°C. It was resistant to oxidizing and reducing agents and the activity was enhanced by detergents. RL74 was 1,3 specific and Km and Vmax for p-nitrophenyl palmitate were 2.73±0.6mM and 101.4±1.9mM/minmg, respectively. N-terminal amino acid sequence showed partial homology with that of Penicillium lipases. RL74 produced biodiesel more efficiently in palm oil than in soybean oil; and the production was highest at pH 8.0, at 5% methanol and at 20% water content.",

keywords = "Biodiesel production, Lipase, Purification, Ralstonia sp. CS274",

author = "Yoo, {Hah Young} and Simkhada, {Jaya Ram} and Cho, {Seung Sik} and Park, {Don Hee} and Kim, {Seung Wook} and Seong, {Chi Nam} and Yoo, {Jin Cheol}",

note = "Funding Information: This work was supported by the Korea Research Foundation (KRF) grant funded by the Korean government (MEST) ( 2010-0029178 ) and Technology Development Program for Agriculture and Forestry , Ministry of Agriculture and Forestry , Republic of Korea, 2007 ( 307014-5 ).",

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doi = "10.1016/j.biortech.2011.02.046",

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AU - Kim, Seung Wook

AU - Seong, Chi Nam

AU - Yoo, Jin Cheol

N1 - Funding Information: This work was supported by the Korea Research Foundation (KRF) grant funded by the Korean government (MEST) ( 2010-0029178 ) and Technology Development Program for Agriculture and Forestry , Ministry of Agriculture and Forestry , Republic of Korea, 2007 ( 307014-5 ).

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N2 - With the aim of isolating a biocatalyst able to catalyze biodiesel production from microbial source, Ralstonia sp. CS274 was isolated and a lipase from the strain (RL74) was purified. Molecular weight of RL74 was estimated to be 28,000Da by SDS-PAGE. The activity was highest at 50-55°C and pH 8.0-9.5 and was stable at pH 7.0-12.0 and up to 45°C. It was resistant to oxidizing and reducing agents and the activity was enhanced by detergents. RL74 was 1,3 specific and Km and Vmax for p-nitrophenyl palmitate were 2.73±0.6mM and 101.4±1.9mM/minmg, respectively. N-terminal amino acid sequence showed partial homology with that of Penicillium lipases. RL74 produced biodiesel more efficiently in palm oil than in soybean oil; and the production was highest at pH 8.0, at 5% methanol and at 20% water content.

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A novel alkaline lipase from Ralstonia with potential application in biodiesel production

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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