Abstract
A Bacillus subtilis promoter, Px, that functions in a convergent manner with the sigA operon promoter P3 has been found in the sigA operon. Promoter Px is turned on at the same time as promoter P3 during early sporulation. The transcript from promoter Px codes for a small protein with partial homology to the OmpR protein from Escherichia coli and also carries an untranslated sequence at its 3' end that is complementary to the 5' end of the P3 transcript, which codes for the ribosome binding site of dnaE. The gene controlled by Px has been called antE. The expression of ante does not require σ,(B) σ(E) or σ(H). Px was transcribed in vitro by the σ(A) holoenzyme and is the seventh promoter to be recognized in the σ(A) operon. A possible role for the antE gene during early sporulation is proposed.
| Original language | English |
|---|---|
| Pages (from-to) | 353-356 |
| Number of pages | 4 |
| Journal | Journal of Bacteriology |
| Volume | 181 |
| Issue number | 1 |
| Publication status | Published - 1999 Jan 1 |
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ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
- Immunology
Cite this
A novel Bacillus subtilis gene, antE, temporally regulated and convergent to and overlapping dnaE. / Wang, Lin F.; Park, Sung Soo; Doi, Roy H.
In: Journal of Bacteriology, Vol. 181, No. 1, 01.01.1999, p. 353-356.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A novel Bacillus subtilis gene, antE, temporally regulated and convergent to and overlapping dnaE
AU - Wang, Lin F.
AU - Park, Sung Soo
AU - Doi, Roy H.
PY - 1999/1/1
Y1 - 1999/1/1
N2 - A Bacillus subtilis promoter, Px, that functions in a convergent manner with the sigA operon promoter P3 has been found in the sigA operon. Promoter Px is turned on at the same time as promoter P3 during early sporulation. The transcript from promoter Px codes for a small protein with partial homology to the OmpR protein from Escherichia coli and also carries an untranslated sequence at its 3' end that is complementary to the 5' end of the P3 transcript, which codes for the ribosome binding site of dnaE. The gene controlled by Px has been called antE. The expression of ante does not require σ,(B) σ(E) or σ(H). Px was transcribed in vitro by the σ(A) holoenzyme and is the seventh promoter to be recognized in the σ(A) operon. A possible role for the antE gene during early sporulation is proposed.
AB - A Bacillus subtilis promoter, Px, that functions in a convergent manner with the sigA operon promoter P3 has been found in the sigA operon. Promoter Px is turned on at the same time as promoter P3 during early sporulation. The transcript from promoter Px codes for a small protein with partial homology to the OmpR protein from Escherichia coli and also carries an untranslated sequence at its 3' end that is complementary to the 5' end of the P3 transcript, which codes for the ribosome binding site of dnaE. The gene controlled by Px has been called antE. The expression of ante does not require σ,(B) σ(E) or σ(H). Px was transcribed in vitro by the σ(A) holoenzyme and is the seventh promoter to be recognized in the σ(A) operon. A possible role for the antE gene during early sporulation is proposed.
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UR - http://www.scopus.com/inward/citedby.url?scp=0032953035&partnerID=8YFLogxK
M3 - Article
C2 - 9864351
AN - SCOPUS:0032953035
VL - 181
SP - 353
EP - 356
JO - Journal of Bacteriology
JF - Journal of Bacteriology
SN - 0021-9193
IS - 1
ER -