A novel chrysin derivative produced by gamma irradiation attenuates 2,4-dinitrochlorobenzene-induced atopic dermatitis-like skin lesions in Balb/c mice

Ha Yeon Song, Woo Sik Kim, Sajid Mushtaq, Ji Min Park, Sung Hyun Choi, Jae Woo Cho, Seung Taik Lim, Eui Baek Byun

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Gamma irradiation is a useful technology to change the physical and biological properties of natural molecules. In this study, we investigated whether gamma irradiation improve properties of chrysin as an anti-inflammatory candidates. Chrysin was converted into two compounds (CM1 and CM2) by gamma irradiation. We determined the therapeutic potential of these compounds in bone marrow-derived macrophages and 2,4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis (AD)-like skin lesions in Balb/c mice. The structural changes to chrysin led to the reduction of cytotoxicity without loss of anti-inflammatory properties in BMDMs. Purified CM2 inhibited lipopolysaccharide (LPS)-induced overexpression of nitric oxide, tumor necrosis factor-α, interleukin (IL)-6, and surface molecules without cytotoxicity in BMDMs, while CM1 revealed strong cytotoxicity. Furthermore, treatment with CM2 significantly alleviated AD-like skin symptoms and clinical signs in DNCB-induced AD mice model. The suppression of AD mediated by CM2 treatment was accompanied by decrease inflammatory T cell cytokines (IFN-γ, IL-5, IL-4, and IL-17). The chemical structure of CM2 and structural transformation mechanism were determined by nuclear magnetic resonance and mass spectrometry. Our study findings provide evidence that CM2 produced by gamma irradiation of chrysin can be an attractive therapeutic agent for AD.

Original languageEnglish
Pages (from-to)223-232
Number of pages10
JournalFood and Chemical Toxicology
Volume128
DOIs
Publication statusPublished - 2019 Jun 1

Keywords

  • Anti-inflammation
  • Atopic dermatitis
  • Chrysin
  • Gamma irradiation
  • Macrophage

ASJC Scopus subject areas

  • Food Science
  • Toxicology

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