A novel cold-adapted lipase, LP28, from a mesophilic Streptomyces strain

Jaya Ram Simkhada, Hah Young Yoo, Seung Sik Cho, Yun Hee Choi, Seung Wook Kim, Don Hee Park, Jin Cheol Yoo

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Fossil fuel is limited but its usage has been growing rapidly, thus the fuel is predicted to be completely running out and causing an unbearable global energy crisis in the near future. To solve this potential crisis, incorporating with increasing environmental concerns, significant attentions have been given to biofuel production in the recent years. With the aim of isolating a microbial biocatalyst with potential application in the field of biofuel, a lipase from Streptomyces sp. CS628, LP28, was purified using hydroxyapatite column chromatography followed by a gel filtration. Molecular weight of LP28 was estimated to be 32,400 Da by SDS-PAGE. The activity was the highest at 30 °C and pH 8.0 and was stable at pH 6.0-8.0 and below 25 °C. The enzyme preferentially hydrolyzed p-nitrophenyl decanoate (C10), a medium chain substrate. Furthermore, LP28 non-specifically hydrolyzed triolein releasing both 1,2- and 1,3-diolein. More importantly, LP28 manifestly catalyzed biodiesel production using palm oil and methanol; therefore, it can be a potential candidate in the field of biofuel.

Original languageEnglish
Pages (from-to)217-225
Number of pages9
JournalBioprocess and Biosystems Engineering
Volume35
Issue number1-2
DOIs
Publication statusPublished - 2012 Jan 1

Fingerprint

Biofuels
Lipases
Streptomyces
Lipase
Column chromatography
Biocatalysts
Palm oil
Decanoates
Fossil Fuels
Biodiesel
Triolein
Hydroxyapatite
Fossil fuels
Methanol
Enzymes
Durapatite
Gels
Molecular weight
Gel Chromatography
Chromatography

Keywords

  • Biodiesel production
  • Cold-adapted lipase
  • Mesophilic
  • Streptomyces

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering

Cite this

Simkhada, J. R., Yoo, H. Y., Cho, S. S., Choi, Y. H., Kim, S. W., Park, D. H., & Yoo, J. C. (2012). A novel cold-adapted lipase, LP28, from a mesophilic Streptomyces strain. Bioprocess and Biosystems Engineering, 35(1-2), 217-225. https://doi.org/10.1007/s00449-011-0597-9

A novel cold-adapted lipase, LP28, from a mesophilic Streptomyces strain. / Simkhada, Jaya Ram; Yoo, Hah Young; Cho, Seung Sik; Choi, Yun Hee; Kim, Seung Wook; Park, Don Hee; Yoo, Jin Cheol.

In: Bioprocess and Biosystems Engineering, Vol. 35, No. 1-2, 01.01.2012, p. 217-225.

Research output: Contribution to journalArticle

Simkhada, Jaya Ram ; Yoo, Hah Young ; Cho, Seung Sik ; Choi, Yun Hee ; Kim, Seung Wook ; Park, Don Hee ; Yoo, Jin Cheol. / A novel cold-adapted lipase, LP28, from a mesophilic Streptomyces strain. In: Bioprocess and Biosystems Engineering. 2012 ; Vol. 35, No. 1-2. pp. 217-225.
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