A novel colorimetric aptasensor using gold nanoparticle for a highly sensitive and specific detection of oxytetracycline

Yeon Seok Kim, Joong Hyun Kim, In Ae Kim, Su Jin Lee, Jongsoo Jurng, Man Bock Gu

Research output: Contribution to journalArticle

135 Citations (Scopus)

Abstract

We have successfully developed a novel colorimetric aptasensor using gold nanoparticles for highly sensitive and specific detection of oxytetracycline (OTC), one of the most common antibacterial agents. A highly specific ssDNA aptamer that bind to OTC with high affinity was employed to discriminate other tetracyclines (TCs), such as doxycycline (DOX) and tetracycline (TET). Aggregation of AuNPs was specifically induced by desorption of the OTC binding aptamers (OBAs) from the surface of gold nanoparticles as a result of the aptamer-target interaction, leading to the color change from red to purple. The detection limit of OTC was enhanced up to 25. nM, which is 20-fold lower than the limit USA-EPA regulated, with two orders of magnitudes in its linear dynamic range by successful optimization on the amount of the aptamers, AuNPs, and salts. This colorimetric aptasensor is advantageous over the other conventional methods in terms of its simple signal generation and detection with the naked eye, which can be realized in on-site detection of antibacterial agents.

Original languageEnglish
Pages (from-to)1644-1649
Number of pages6
JournalBiosensors and Bioelectronics
Volume26
Issue number4
DOIs
Publication statusPublished - 2010 Dec 15

Fingerprint

Bactericides
Oxytetracycline
Gold
Nanoparticles
Desorption
Agglomeration
Salts
Anti-Bacterial Agents
Color
Tetracyclines
Doxycycline
Tetracycline
Limit of Detection

Keywords

  • Aptamers
  • Colorimetric detection
  • Gold nanoparticles
  • Oxytetracyclines

ASJC Scopus subject areas

  • Biophysics
  • Biomedical Engineering
  • Biotechnology
  • Electrochemistry

Cite this

A novel colorimetric aptasensor using gold nanoparticle for a highly sensitive and specific detection of oxytetracycline. / Kim, Yeon Seok; Kim, Joong Hyun; Kim, In Ae; Lee, Su Jin; Jurng, Jongsoo; Gu, Man Bock.

In: Biosensors and Bioelectronics, Vol. 26, No. 4, 15.12.2010, p. 1644-1649.

Research output: Contribution to journalArticle

Kim, Yeon Seok ; Kim, Joong Hyun ; Kim, In Ae ; Lee, Su Jin ; Jurng, Jongsoo ; Gu, Man Bock. / A novel colorimetric aptasensor using gold nanoparticle for a highly sensitive and specific detection of oxytetracycline. In: Biosensors and Bioelectronics. 2010 ; Vol. 26, No. 4. pp. 1644-1649.
@article{ccb9a4a09b534c67bcd5dec3e7e638fc,
title = "A novel colorimetric aptasensor using gold nanoparticle for a highly sensitive and specific detection of oxytetracycline",
abstract = "We have successfully developed a novel colorimetric aptasensor using gold nanoparticles for highly sensitive and specific detection of oxytetracycline (OTC), one of the most common antibacterial agents. A highly specific ssDNA aptamer that bind to OTC with high affinity was employed to discriminate other tetracyclines (TCs), such as doxycycline (DOX) and tetracycline (TET). Aggregation of AuNPs was specifically induced by desorption of the OTC binding aptamers (OBAs) from the surface of gold nanoparticles as a result of the aptamer-target interaction, leading to the color change from red to purple. The detection limit of OTC was enhanced up to 25. nM, which is 20-fold lower than the limit USA-EPA regulated, with two orders of magnitudes in its linear dynamic range by successful optimization on the amount of the aptamers, AuNPs, and salts. This colorimetric aptasensor is advantageous over the other conventional methods in terms of its simple signal generation and detection with the naked eye, which can be realized in on-site detection of antibacterial agents.",
keywords = "Aptamers, Colorimetric detection, Gold nanoparticles, Oxytetracyclines",
author = "Kim, {Yeon Seok} and Kim, {Joong Hyun} and Kim, {In Ae} and Lee, {Su Jin} and Jongsoo Jurng and Gu, {Man Bock}",
year = "2010",
month = "12",
day = "15",
doi = "10.1016/j.bios.2010.08.046",
language = "English",
volume = "26",
pages = "1644--1649",
journal = "Biosensors",
issn = "0956-5663",
publisher = "Elsevier Limited",
number = "4",

}

TY - JOUR

T1 - A novel colorimetric aptasensor using gold nanoparticle for a highly sensitive and specific detection of oxytetracycline

AU - Kim, Yeon Seok

AU - Kim, Joong Hyun

AU - Kim, In Ae

AU - Lee, Su Jin

AU - Jurng, Jongsoo

AU - Gu, Man Bock

PY - 2010/12/15

Y1 - 2010/12/15

N2 - We have successfully developed a novel colorimetric aptasensor using gold nanoparticles for highly sensitive and specific detection of oxytetracycline (OTC), one of the most common antibacterial agents. A highly specific ssDNA aptamer that bind to OTC with high affinity was employed to discriminate other tetracyclines (TCs), such as doxycycline (DOX) and tetracycline (TET). Aggregation of AuNPs was specifically induced by desorption of the OTC binding aptamers (OBAs) from the surface of gold nanoparticles as a result of the aptamer-target interaction, leading to the color change from red to purple. The detection limit of OTC was enhanced up to 25. nM, which is 20-fold lower than the limit USA-EPA regulated, with two orders of magnitudes in its linear dynamic range by successful optimization on the amount of the aptamers, AuNPs, and salts. This colorimetric aptasensor is advantageous over the other conventional methods in terms of its simple signal generation and detection with the naked eye, which can be realized in on-site detection of antibacterial agents.

AB - We have successfully developed a novel colorimetric aptasensor using gold nanoparticles for highly sensitive and specific detection of oxytetracycline (OTC), one of the most common antibacterial agents. A highly specific ssDNA aptamer that bind to OTC with high affinity was employed to discriminate other tetracyclines (TCs), such as doxycycline (DOX) and tetracycline (TET). Aggregation of AuNPs was specifically induced by desorption of the OTC binding aptamers (OBAs) from the surface of gold nanoparticles as a result of the aptamer-target interaction, leading to the color change from red to purple. The detection limit of OTC was enhanced up to 25. nM, which is 20-fold lower than the limit USA-EPA regulated, with two orders of magnitudes in its linear dynamic range by successful optimization on the amount of the aptamers, AuNPs, and salts. This colorimetric aptasensor is advantageous over the other conventional methods in terms of its simple signal generation and detection with the naked eye, which can be realized in on-site detection of antibacterial agents.

KW - Aptamers

KW - Colorimetric detection

KW - Gold nanoparticles

KW - Oxytetracyclines

UR - http://www.scopus.com/inward/record.url?scp=78649748231&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78649748231&partnerID=8YFLogxK

U2 - 10.1016/j.bios.2010.08.046

DO - 10.1016/j.bios.2010.08.046

M3 - Article

VL - 26

SP - 1644

EP - 1649

JO - Biosensors

JF - Biosensors

SN - 0956-5663

IS - 4

ER -