A novel culture model for human pluripotent stem cell propagation on gelatin in placenta-conditioned media

Ji Hye Jung, Byung Soo Kim

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The propagation of human pluripotent stem cells (hPSCs) in conditioned medium derived from human cells in feeder-free culture conditions has been of interest. Nevertheless, an ideal humanized ex vivo feeder-free propagation method for hPSCs has not been developed; currently, additional exogenous substrates including basic fibroblast growth factor (bFGF), a master hPSC-sustaining factor, is added to all of culture media and synthetic substrata such as Matrigel or laminin are used in all feeder-free cultures. Recently, our group developed a simple and efficient protocol for the propagation of hPSCs using only conditioned media derived from the human placenta on a gelatin-coated dish without additional exogenous supplementation or synthetic substrata specific to hPSCs. This protocol has not been reported previously and might enable researchers to propagate hPSCs efficiently in humanized culture conditions. Additionally, this model obviates hPSC contamination risks by animal products such as viruses or unknown proteins. Furthermore, this system facilitates easy mass production of hPSCs using the gelatine coating, which is simple to handle, dramatically decreases the overall costs of ex vivo hPSC maintenance.

Original languageEnglish
Article numbere53204
JournalJournal of Visualized Experiments
Volume2015
Issue number102
DOIs
Publication statusPublished - 2015 Aug 3

Fingerprint

Pluripotent Stem Cells
Gelatin
Conditioned Culture Medium
Stem cells
Cell culture
Placenta
Stem Cell Factor
Laminin
Fibroblast Growth Factor 2
Viruses
Feeder Cells
Fibroblasts
Culture Media
Animals
Contamination
Cells
Coatings
Proteins
Maintenance
Research Personnel

Keywords

  • BFGF
  • Developmental biology
  • Human embryonic stem cells
  • Human placenta chorionic villi
  • Induced-pluripotent stem cells
  • Stem cell biology
  • Synthetic substrates

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemical Engineering(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)

Cite this

A novel culture model for human pluripotent stem cell propagation on gelatin in placenta-conditioned media. / Jung, Ji Hye; Kim, Byung Soo.

In: Journal of Visualized Experiments, Vol. 2015, No. 102, e53204, 03.08.2015.

Research output: Contribution to journalArticle

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