A novel enzyme-immobilization method for a biofuel cell

Jin Young Lee, Hyun Yong Shin, Jong H. Lee, Yoon Seok Song, Seong Woo Kang, Chulhwan Park, Jungbae Kim, Seung Wook Kim

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Biofuel cells utilizing biocatalysts are attractive alternatives to metal catalyst-based cells because of environmentally friendly cells and their renewability and good operations at room temperatures, even though they provide a low level of electrical power. In this study, the effect of a novel enzyme immobilization method on anodic electrical properties was evaluated under ambient conditions for increasing the power of an enzyme-based biofuel cell. The anodic system employed in the cell contained a gold electrode, pyrroloquinoline quinone (PQQ) as the electron transfer mediator, lactate dehydrogenase (LDH), β-nicotinamide adenine dinucleotide (NAD+) as the cofactor, and lactate as the substrate. The anodic electrical properties increased as a result of the novel enzyme-immobilization method. Furthermore, lactate, NAD+, or CaCl2, which can all influence enzyme activation, were used to prevent covalent bond formation near the active site of the LDH during enzyme-immobilization. Protection of the active site of the LDH using this novel enzyme-immobilization method increased its stability, which enabled to increase power production (142 μW/cm2) in a basic enzymatic fuel cell (EFC).

Original languageEnglish
Pages (from-to)274-278
Number of pages5
JournalJournal of Molecular Catalysis B: Enzymatic
Volume59
Issue number4
DOIs
Publication statusPublished - 2009 Aug 1

Fingerprint

Bioelectric Energy Sources
Biological fuel cells
Enzyme immobilization
Immobilization
L-Lactate Dehydrogenase
NAD
Enzymes
Lactic Acid
Enzymatic fuel cells
Electric properties
PQQ Cofactor
Biocatalysts
Covalent bonds
Catalytic Domain
Gold
Enzyme Activation
Metals
Chemical activation
Electrodes
Catalysts

Keywords

  • β-Nicotinamide adenine dinucleotide
  • Enzyme stability
  • Immobilization
  • Lactate dehydrogenase
  • Pyrroloquinoline quinone

ASJC Scopus subject areas

  • Biochemistry
  • Bioengineering
  • Catalysis
  • Process Chemistry and Technology

Cite this

A novel enzyme-immobilization method for a biofuel cell. / Lee, Jin Young; Shin, Hyun Yong; Lee, Jong H.; Song, Yoon Seok; Kang, Seong Woo; Park, Chulhwan; Kim, Jungbae; Kim, Seung Wook.

In: Journal of Molecular Catalysis B: Enzymatic, Vol. 59, No. 4, 01.08.2009, p. 274-278.

Research output: Contribution to journalArticle

Lee, Jin Young ; Shin, Hyun Yong ; Lee, Jong H. ; Song, Yoon Seok ; Kang, Seong Woo ; Park, Chulhwan ; Kim, Jungbae ; Kim, Seung Wook. / A novel enzyme-immobilization method for a biofuel cell. In: Journal of Molecular Catalysis B: Enzymatic. 2009 ; Vol. 59, No. 4. pp. 274-278.
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