A novel extensin gene encoding a hydroxyproline-rich glycoprotein requires sucrose for its wound-inducible expression in transgenic plants

Ji Hoon Ahn, Yeonhee Choi, Young Myung Kwon, Sang Gu Kim, Yang Do Choi, Jong Seob Lee

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

A novel hydroxyproline-rich glycoprotein (SbHRGP3) that consists of two different domains is encoded by an extensin gene from soybean. The first domain (domain 1) located at the N terminus is composed of 11 repeats of Ser-Pro 4-Lys-His-Ser-Pro 4-Tyr 3-His, whereas the second domain (domain 2) at the C terminus contains five repeats of Ser-Pro 4-Val-Tyr-Lys-Tyr-Lys- Ser-Pro 4-Tyr-Lys-Tyr-Pro-Ser-Pro 5-Tyr-Lys-Tyr-Pro-Ser-Pro 4-Val -Tyr-Lys- Tyr-Lys. These two repeat motifs are organized in an extremely well-ordered pattern in each domain, which suggests that SbHRGP3 belongs to s new group of proteins having the repeat motifs of two distinct groups of dicot extensins. The expression of the SbHRGP3 gene increased with seedling maturation, and its expression was relatively high in the mature regions of the hypocotyl and in the root of soybean seedlings. An SbHRGP3-β- glucuronidase (SbHRGP3-GUS) chimeric gene was constructed and expressed in transgenic tobacco plants. The expression of the SbHRGP3-GUS gene was not induced by wounding alone in transgenic tobacco plants; sucrose was also required. Expression was specific to phloem tissues and cambium cells of leaves and stems. In transgenic tobacco seedlings, SbHRGP3-GUS gene expression was activated by the maturation of the primary root and then inactivated; however, reactivation was specifically st the epidermis of the zone from which the lateral root was to be initiated. Its reactivation occurred just before the lateral root initiation. These results indicate that the SbHRGP3 gene in different tissues responds to different signals.

Original languageEnglish
Pages (from-to)1477-1490
Number of pages14
JournalPlant Cell
Volume8
Issue number9
DOIs
Publication statusPublished - 1996 Sep 1
Externally publishedYes

Fingerprint

extensin
Gene encoding
hydroxyproline
Genetically Modified Plants
Hydroxyproline
plant damage
seryl-proline
Sucrose
glycoproteins
transgenic plants
Glucuronidase
Glycoproteins
Genes
Seedlings
sucrose
Tobacco
tobacco
Wounds and Injuries
genetically modified organisms
Soybeans

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

A novel extensin gene encoding a hydroxyproline-rich glycoprotein requires sucrose for its wound-inducible expression in transgenic plants. / Ahn, Ji Hoon; Choi, Yeonhee; Kwon, Young Myung; Kim, Sang Gu; Choi, Yang Do; Lee, Jong Seob.

In: Plant Cell, Vol. 8, No. 9, 01.09.1996, p. 1477-1490.

Research output: Contribution to journalArticle

Ahn, Ji Hoon ; Choi, Yeonhee ; Kwon, Young Myung ; Kim, Sang Gu ; Choi, Yang Do ; Lee, Jong Seob. / A novel extensin gene encoding a hydroxyproline-rich glycoprotein requires sucrose for its wound-inducible expression in transgenic plants. In: Plant Cell. 1996 ; Vol. 8, No. 9. pp. 1477-1490.
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