A novel F-box protein represses endothecial secondary wall thickening for anther dehiscence in Arabidopsis thaliana

Yun Young Kim, Kwang Wook Jung, Ji Ung Jeung, Jeong Sheop Shin

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

In plants, the regulation of protein turnover by the ubiquitin proteasome system (UPS) is a key posttranslational mechanism underlying diverse cellular processes. However, the participation of the UPS in cellular processes involved in anther dehiscence, especially endothecial secondary wall (ESW) thickening, has not been characterized. Here, we report that a novel F-box protein in arabidopsis, designated SAF1 (Secondary wall thickening-Associated F-box 1), negatively regulates ESW thickening in the anther. SAF1 is predominantly expressed in flower tissues and interacts with Arabidopsis-Skp1-like 19 (ASK19). SAF1-overexpressed (Ox) lines showed reduced fertility due to a lack or loss of ESW thickening in the anther and inhibition of the expression of relevant genes, such as IRREGURAR XYLEMs (IRXs) in flowers. These findings suggest that the novel Skp/Cul/F-box (SCF) complex consisting of SAF1 as an F-box protein and ASK19 as a Skp functions in secondary wall thickening of the anther endothecium.

Original languageEnglish
Pages (from-to)212-216
Number of pages5
JournalJournal of Plant Physiology
Volume169
Issue number2
DOIs
Publication statusPublished - 2012 Jan 15

Fingerprint

F-Box Proteins
F-box proteins
dehiscence
Arabidopsis
anthers
Arabidopsis thaliana
Proteasome Endopeptidase Complex
Ubiquitin
proteasome endopeptidase complex
ubiquitin
Plant Proteins
flowers
Fertility
protein metabolism
Gene Expression
gene expression

Keywords

  • Anther dehiscence
  • Arabidopsis
  • ASK
  • Endothecial secondary wall thickening
  • F-box protein

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Agronomy and Crop Science

Cite this

A novel F-box protein represses endothecial secondary wall thickening for anther dehiscence in Arabidopsis thaliana. / Kim, Yun Young; Jung, Kwang Wook; Jeung, Ji Ung; Shin, Jeong Sheop.

In: Journal of Plant Physiology, Vol. 169, No. 2, 15.01.2012, p. 212-216.

Research output: Contribution to journalArticle

@article{ef6b915e506a4d5c8f7813f0f94602c4,
title = "A novel F-box protein represses endothecial secondary wall thickening for anther dehiscence in Arabidopsis thaliana",
abstract = "In plants, the regulation of protein turnover by the ubiquitin proteasome system (UPS) is a key posttranslational mechanism underlying diverse cellular processes. However, the participation of the UPS in cellular processes involved in anther dehiscence, especially endothecial secondary wall (ESW) thickening, has not been characterized. Here, we report that a novel F-box protein in arabidopsis, designated SAF1 (Secondary wall thickening-Associated F-box 1), negatively regulates ESW thickening in the anther. SAF1 is predominantly expressed in flower tissues and interacts with Arabidopsis-Skp1-like 19 (ASK19). SAF1-overexpressed (Ox) lines showed reduced fertility due to a lack or loss of ESW thickening in the anther and inhibition of the expression of relevant genes, such as IRREGURAR XYLEMs (IRXs) in flowers. These findings suggest that the novel Skp/Cul/F-box (SCF) complex consisting of SAF1 as an F-box protein and ASK19 as a Skp functions in secondary wall thickening of the anther endothecium.",
keywords = "Anther dehiscence, Arabidopsis, ASK, Endothecial secondary wall thickening, F-box protein",
author = "Kim, {Yun Young} and Jung, {Kwang Wook} and Jeung, {Ji Ung} and Shin, {Jeong Sheop}",
year = "2012",
month = "1",
day = "15",
doi = "10.1016/j.jplph.2011.09.006",
language = "English",
volume = "169",
pages = "212--216",
journal = "Journal of Plant Physiology",
issn = "0176-1617",
publisher = "Urban und Fischer Verlag GmbH und Co. KG",
number = "2",

}

TY - JOUR

T1 - A novel F-box protein represses endothecial secondary wall thickening for anther dehiscence in Arabidopsis thaliana

AU - Kim, Yun Young

AU - Jung, Kwang Wook

AU - Jeung, Ji Ung

AU - Shin, Jeong Sheop

PY - 2012/1/15

Y1 - 2012/1/15

N2 - In plants, the regulation of protein turnover by the ubiquitin proteasome system (UPS) is a key posttranslational mechanism underlying diverse cellular processes. However, the participation of the UPS in cellular processes involved in anther dehiscence, especially endothecial secondary wall (ESW) thickening, has not been characterized. Here, we report that a novel F-box protein in arabidopsis, designated SAF1 (Secondary wall thickening-Associated F-box 1), negatively regulates ESW thickening in the anther. SAF1 is predominantly expressed in flower tissues and interacts with Arabidopsis-Skp1-like 19 (ASK19). SAF1-overexpressed (Ox) lines showed reduced fertility due to a lack or loss of ESW thickening in the anther and inhibition of the expression of relevant genes, such as IRREGURAR XYLEMs (IRXs) in flowers. These findings suggest that the novel Skp/Cul/F-box (SCF) complex consisting of SAF1 as an F-box protein and ASK19 as a Skp functions in secondary wall thickening of the anther endothecium.

AB - In plants, the regulation of protein turnover by the ubiquitin proteasome system (UPS) is a key posttranslational mechanism underlying diverse cellular processes. However, the participation of the UPS in cellular processes involved in anther dehiscence, especially endothecial secondary wall (ESW) thickening, has not been characterized. Here, we report that a novel F-box protein in arabidopsis, designated SAF1 (Secondary wall thickening-Associated F-box 1), negatively regulates ESW thickening in the anther. SAF1 is predominantly expressed in flower tissues and interacts with Arabidopsis-Skp1-like 19 (ASK19). SAF1-overexpressed (Ox) lines showed reduced fertility due to a lack or loss of ESW thickening in the anther and inhibition of the expression of relevant genes, such as IRREGURAR XYLEMs (IRXs) in flowers. These findings suggest that the novel Skp/Cul/F-box (SCF) complex consisting of SAF1 as an F-box protein and ASK19 as a Skp functions in secondary wall thickening of the anther endothecium.

KW - Anther dehiscence

KW - Arabidopsis

KW - ASK

KW - Endothecial secondary wall thickening

KW - F-box protein

UR - http://www.scopus.com/inward/record.url?scp=84355162128&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84355162128&partnerID=8YFLogxK

U2 - 10.1016/j.jplph.2011.09.006

DO - 10.1016/j.jplph.2011.09.006

M3 - Article

C2 - 22018967

AN - SCOPUS:84355162128

VL - 169

SP - 212

EP - 216

JO - Journal of Plant Physiology

JF - Journal of Plant Physiology

SN - 0176-1617

IS - 2

ER -