A novel fed-batch process based on the biology of Aurantiochytrium sp. KRS101 for the production of biodiesel and docosahexaenoic acid

Kyochan Kim, Eun Jung Kim, Byung Gon Ryu, Soojung Park, Yoon-E Choi, Ji Won Yang

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The biology of Aurantiochytrium sp. KRS101 was thoroughly investigated to enhance its production of biodiesel and docosahexaenoic acid (DHA). Nutrients and salinity were optimized to prevent biomass loss due to cell rupture. Calculation of yield coefficients showed that nitrogen was mostly responsible for the early stage of cell growth or division, whereas carbon was necessary for the entire process of cell development, particularly cell enlargement during late stages. Using these distinctive yield coefficients, a modified fed-batch cultivation method was designed, resulting in increases in palmitic acid (PA) and DHA production of up to 137% and 29%, respectively. This modified fed-batch cultivation method, using appropriate supplies of nitrogen and carbon, may improve the yields of PA and DHA, thus expanding the biotechnological applications of Aurantiochytrium sp. KRS101.

Original languageEnglish
Pages (from-to)269-274
Number of pages6
JournalBioresource Technology
Volume135
DOIs
Publication statusPublished - 2013 Jan 1
Externally publishedYes

Fingerprint

Biofuels
Docosahexaenoic Acids
Biodiesel
Palmitic acid
Palmitic Acid
Acids
acid
Nitrogen
Carbon
Cell growth
Nutrients
Biomass
nitrogen
carbon
rupture
batch process
salinity
nutrient
biomass

Keywords

  • Aurantiochytrium sp.
  • Biodiesel
  • Docosahexaenoic acid
  • Modified fed-batch
  • Yield coefficients

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

Cite this

A novel fed-batch process based on the biology of Aurantiochytrium sp. KRS101 for the production of biodiesel and docosahexaenoic acid. / Kim, Kyochan; Jung Kim, Eun; Ryu, Byung Gon; Park, Soojung; Choi, Yoon-E; Yang, Ji Won.

In: Bioresource Technology, Vol. 135, 01.01.2013, p. 269-274.

Research output: Contribution to journalArticle

Kim, Kyochan ; Jung Kim, Eun ; Ryu, Byung Gon ; Park, Soojung ; Choi, Yoon-E ; Yang, Ji Won. / A novel fed-batch process based on the biology of Aurantiochytrium sp. KRS101 for the production of biodiesel and docosahexaenoic acid. In: Bioresource Technology. 2013 ; Vol. 135. pp. 269-274.
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