A novel function of Aft1 in regulating ferrioxamine B uptake: Aft1 modulates Arn3 ubiquitination in Saccharomyces cerevisiae

Mi Young Jeong, Chang Min Kang, Ji Hyun Kim, Dong Hyuk Heo, Miwha Chang, In Joon Baek, Hyeong Su Ro, Il Dong Choi, Tae Hyoung Kim, Cheol-Won Yun

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Aft1 is a transcriptional activator in Saccharomyces cerevisiae that responds to iron availability and regulates the expression of genes in the iron regulon, such as FET3, FTR1 and the ARN family. Using a two-hybrid screen, we found that Aft1 physically interacts with the FOB (ferrioxamine B) transporter Arn3. This interaction modulates the ability of Arn3 to take up FOB. The interaction between Arn3 and Aft1 was confirmed by β-galactosidase, co-immunoprecipitation and SPR (surface plasmon resonance) assays. Truncated Aft1 had a stronger interaction with Arn3 and caused a higher FOB-uptake activity than full-length Aft1. Interestingly, only full-length Aft1 induced the correct localization of Arn3 in response to FOB. Furthermore, we found Aft1 affected Arn3 ubiquitination. These results suggest that Aft1 interacts with Arn3 and may regulate the ubiquitination of Arn3 in the cytosolic compartment.

Original languageEnglish
Pages (from-to)181-191
Number of pages11
JournalBiochemical Journal
Volume422
Issue number1
DOIs
Publication statusPublished - 2009 Aug 15

Keywords

  • Aft1
  • Arn3
  • Iron
  • Saccharomyces cerevisiae
  • Siderophore
  • Ubiquitination

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

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    Jeong, M. Y., Kang, C. M., Kim, J. H., Heo, D. H., Chang, M., Baek, I. J., Ro, H. S., Choi, I. D., Kim, T. H., & Yun, C-W. (2009). A novel function of Aft1 in regulating ferrioxamine B uptake: Aft1 modulates Arn3 ubiquitination in Saccharomyces cerevisiae. Biochemical Journal, 422(1), 181-191. https://doi.org/10.1042/BJ20082399