A novel glycoside hydrolase family 5 β-1,3-1,6-endoglucanase from Saccharophagus degradans 2-40T and its transglycosylase activity

Damao Wang, Do Hyoung Kim, Nari Seo, Eun Ju Yun, Hyun Joo An, Jae Han Kim, Kyoung Heon Kim

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

In this study, we characterized Gly5M, originating from a marine bacterium, as a novel β-1,3-1,6-endoglucanase in glycoside hydrolase family 5 (GH5) in the Carbohydrate-Active enZyme database. The gly5M gene encodes Gly5M, a newly characterized enzyme from GH5 subfamily 47 (GH5_47) in Saccharophagus degradans 2-40T. The gly5M gene was cloned and overexpressed in Escherichia coli. Through analysis of the enzymatic reaction products by thin-layer chromatography, high-performance liquid chromatography, and matrix-assisted laser desorption ionization-tandem time of flight mass spectrometry, Gly5M was identified as a novel β-1,3-endoglucanase (EC 3.2.1.39) and bacterial β-1,6-glucanase (EC 3.2.1.75) in GH5. The β-1,3-endoglucanase and β-1,6-endoglucanase activities were detected by using laminarin (a β-1,3- glucan with β-1,6-glycosidic linkages derived from brown macroalgae) and pustulan (a β-1,6-glucan derived from fungal cell walls) as the substrates, respectively. This enzyme also showed transglycosylase activity toward β-1,3-oligosaccharides when laminarioligosaccharides were used as the substrates. Since laminarin is the major form of glucan storage in brown macroalgae, Gly5M could be used to produce glucose and laminarioligosaccharides, using brown macroalgae, for industrial purposes.

Original languageEnglish
Pages (from-to)4340-4349
Number of pages10
JournalApplied and Environmental Microbiology
Volume82
Issue number14
DOIs
Publication statusPublished - 2016

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Cellulase
Glycoside Hydrolases
hydrolases
endo-1,4-beta-glucanase
Seaweed
Glucans
glycosides
glucans
enzyme
macroalgae
endo-1,6-beta-glucanase
substrate
Enzymes
enzymes
gene
glycosidic linkages
chromatography
liquid chromatography
carbohydrate
desorption

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Food Science
  • Biotechnology
  • Ecology

Cite this

A novel glycoside hydrolase family 5 β-1,3-1,6-endoglucanase from Saccharophagus degradans 2-40T and its transglycosylase activity. / Wang, Damao; Kim, Do Hyoung; Seo, Nari; Yun, Eun Ju; An, Hyun Joo; Kim, Jae Han; Kim, Kyoung Heon.

In: Applied and Environmental Microbiology, Vol. 82, No. 14, 2016, p. 4340-4349.

Research output: Contribution to journalArticle

Wang, Damao ; Kim, Do Hyoung ; Seo, Nari ; Yun, Eun Ju ; An, Hyun Joo ; Kim, Jae Han ; Kim, Kyoung Heon. / A novel glycoside hydrolase family 5 β-1,3-1,6-endoglucanase from Saccharophagus degradans 2-40T and its transglycosylase activity. In: Applied and Environmental Microbiology. 2016 ; Vol. 82, No. 14. pp. 4340-4349.
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AU - An, Hyun Joo

AU - Kim, Jae Han

AU - Kim, Kyoung Heon

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