TY - JOUR
T1 - A novel helper qPCR system for platinum detection via Pt-DNA coordination
AU - Cai, Sheng
AU - Yang, Dan
AU - Tian, Xueke
AU - Ye, Jiawei
AU - Xu, Mingcheng
AU - Abdullah AL-maskri, Abdu Ahmed
AU - Jung, Cheulhee
AU - Zeng, Su
N1 - Funding Information:
We acknowledge financial support from the National Key Project of China ( 2017YFC0908600 ), the National Natural Science Foundation of China (Grant 21405136 ), a Korea University Grant, the Zhejiang Provincial Natural Science Foundation of China ( LY18H300003 ), Scientific Research Fund of Zhejiang Provincial Education Department ( N20140206 ). We thank Ms. Haihong Hu for managing instruments and helping experiments.
Funding Information:
We acknowledge financial support from the National Key Project of China (2017YFC0908600), the National Natural Science Foundation of China (Grant 21405136), a Korea University Grant, the Zhejiang Provincial Natural Science Foundation of China (LY18H300003), Scientific Research Fund of Zhejiang Provincial Education Department (N20140206). We thank Ms. Haihong Hu for managing instruments and helping experiments.
Publisher Copyright:
© 2018 Elsevier B.V.
Copyright:
Copyright 2019 Elsevier B.V., All rights reserved.
PY - 2019/3/7
Y1 - 2019/3/7
N2 - A novel real-time polymerase chain reaction (qPCR) platform for the simple and robust detection of platinum is described for the first time. Compared with conventional qPCR, a helper template, which is related to the active template for performing qPCR, was introduced in our helper qPCR system. Several guanine (G) bases were introduced in the helper template to obtain a platinum-responsive on/off switch based on G-Pt-G coordination. Because of the helper template, a slight change in platinum concentration would significantly change the signal in the qPCR. This novel helper qPCR technique easily detects platinum with high sensitivity (1 ng/mL) and selectivity over other metal ions. Therefore, it will be a promising technique for the detection of platinum in biomedical and environmental samples.
AB - A novel real-time polymerase chain reaction (qPCR) platform for the simple and robust detection of platinum is described for the first time. Compared with conventional qPCR, a helper template, which is related to the active template for performing qPCR, was introduced in our helper qPCR system. Several guanine (G) bases were introduced in the helper template to obtain a platinum-responsive on/off switch based on G-Pt-G coordination. Because of the helper template, a slight change in platinum concentration would significantly change the signal in the qPCR. This novel helper qPCR technique easily detects platinum with high sensitivity (1 ng/mL) and selectivity over other metal ions. Therefore, it will be a promising technique for the detection of platinum in biomedical and environmental samples.
KW - Helper qPCR platform
KW - Platinum detection
KW - Platinum-responsive on/off switch
KW - Pt-DNA coordination
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U2 - 10.1016/j.aca.2018.11.007
DO - 10.1016/j.aca.2018.11.007
M3 - Article
C2 - 30661583
AN - SCOPUS:85056818537
VL - 1050
SP - 154
EP - 160
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
SN - 0003-2670
ER -