A novel helper qPCR system for platinum detection via Pt-DNA coordination

Sheng Cai, Dan Yang, Xueke Tian, Jiawei Ye, Mingcheng Xu, Abdu Ahmed Abdullah AL-maskri, Cheulhee Jung, Su Zeng

Research output: Contribution to journalArticle

Abstract

A novel real-time polymerase chain reaction (qPCR) platform for the simple and robust detection of platinum is described for the first time. Compared with conventional qPCR, a helper template, which is related to the active template for performing qPCR, was introduced in our helper qPCR system. Several guanine (G) bases were introduced in the helper template to obtain a platinum-responsive on/off switch based on G-Pt-G coordination. Because of the helper template, a slight change in platinum concentration would significantly change the signal in the qPCR. This novel helper qPCR technique easily detects platinum with high sensitivity (1 ng/mL) and selectivity over other metal ions. Therefore, it will be a promising technique for the detection of platinum in biomedical and environmental samples.

Original languageEnglish
Pages (from-to)154-160
Number of pages7
JournalAnalytica Chimica Acta
Volume1050
DOIs
Publication statusPublished - 2019 Mar 7

Fingerprint

Platinum
platinum
DNA
Polymerase chain reaction
Guanine
polymerase chain reaction
Metal ions
Real-Time Polymerase Chain Reaction
Metals
Switches
co-ordination
detection
Ions
ion
metal

Keywords

  • Helper qPCR platform
  • Platinum detection
  • Platinum-responsive on/off switch
  • Pt-DNA coordination

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

Cite this

Cai, S., Yang, D., Tian, X., Ye, J., Xu, M., Abdullah AL-maskri, A. A., ... Zeng, S. (2019). A novel helper qPCR system for platinum detection via Pt-DNA coordination. Analytica Chimica Acta, 1050, 154-160. https://doi.org/10.1016/j.aca.2018.11.007

A novel helper qPCR system for platinum detection via Pt-DNA coordination. / Cai, Sheng; Yang, Dan; Tian, Xueke; Ye, Jiawei; Xu, Mingcheng; Abdullah AL-maskri, Abdu Ahmed; Jung, Cheulhee; Zeng, Su.

In: Analytica Chimica Acta, Vol. 1050, 07.03.2019, p. 154-160.

Research output: Contribution to journalArticle

Cai, S, Yang, D, Tian, X, Ye, J, Xu, M, Abdullah AL-maskri, AA, Jung, C & Zeng, S 2019, 'A novel helper qPCR system for platinum detection via Pt-DNA coordination', Analytica Chimica Acta, vol. 1050, pp. 154-160. https://doi.org/10.1016/j.aca.2018.11.007
Cai S, Yang D, Tian X, Ye J, Xu M, Abdullah AL-maskri AA et al. A novel helper qPCR system for platinum detection via Pt-DNA coordination. Analytica Chimica Acta. 2019 Mar 7;1050:154-160. https://doi.org/10.1016/j.aca.2018.11.007
Cai, Sheng ; Yang, Dan ; Tian, Xueke ; Ye, Jiawei ; Xu, Mingcheng ; Abdullah AL-maskri, Abdu Ahmed ; Jung, Cheulhee ; Zeng, Su. / A novel helper qPCR system for platinum detection via Pt-DNA coordination. In: Analytica Chimica Acta. 2019 ; Vol. 1050. pp. 154-160.
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