A novel helper qPCR system for platinum detection via Pt-DNA coordination

Sheng Cai; Dan Yang; Xueke Tian; Jiawei Ye; Mingcheng Xu; Abdu Ahmed Abdullah AL-maskri; Cheulhee Jung; Su Zeng

doi:10.1016/j.aca.2018.11.007

A novel helper qPCR system for platinum detection via Pt-DNA coordination

Sheng Cai, Dan Yang, Xueke Tian, Jiawei Ye, Mingcheng Xu, Abdu Ahmed Abdullah AL-maskri, Cheulhee Jung, Su Zeng

Research output: Contribution to journal › Article

1 Citation (Scopus)

Abstract

A novel real-time polymerase chain reaction (qPCR) platform for the simple and robust detection of platinum is described for the first time. Compared with conventional qPCR, a helper template, which is related to the active template for performing qPCR, was introduced in our helper qPCR system. Several guanine (G) bases were introduced in the helper template to obtain a platinum-responsive on/off switch based on G-Pt-G coordination. Because of the helper template, a slight change in platinum concentration would significantly change the signal in the qPCR. This novel helper qPCR technique easily detects platinum with high sensitivity (1 ng/mL) and selectivity over other metal ions. Therefore, it will be a promising technique for the detection of platinum in biomedical and environmental samples.

Original language	English
Pages (from-to)	154-160
Number of pages	7
Journal	Analytica Chimica Acta
Volume	1050
DOIs	https://doi.org/10.1016/j.aca.2018.11.007
Publication status	Published - 2019 Mar 7

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Keywords

Helper qPCR platform
Platinum detection
Platinum-responsive on/off switch
Pt-DNA coordination

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Environmental Chemistry
Spectroscopy

Cite this

Cai, S., Yang, D., Tian, X., Ye, J., Xu, M., Abdullah AL-maskri, A. A., Jung, C., & Zeng, S. (2019). A novel helper qPCR system for platinum detection via Pt-DNA coordination. Analytica Chimica Acta, 1050, 154-160. https://doi.org/10.1016/j.aca.2018.11.007

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abstract = "A novel real-time polymerase chain reaction (qPCR) platform for the simple and robust detection of platinum is described for the first time. Compared with conventional qPCR, a helper template, which is related to the active template for performing qPCR, was introduced in our helper qPCR system. Several guanine (G) bases were introduced in the helper template to obtain a platinum-responsive on/off switch based on G-Pt-G coordination. Because of the helper template, a slight change in platinum concentration would significantly change the signal in the qPCR. This novel helper qPCR technique easily detects platinum with high sensitivity (1 ng/mL) and selectivity over other metal ions. Therefore, it will be a promising technique for the detection of platinum in biomedical and environmental samples.",

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AU - Tian, Xueke

AU - Ye, Jiawei

AU - Xu, Mingcheng

AU - Abdullah AL-maskri, Abdu Ahmed

AU - Jung, Cheulhee

AU - Zeng, Su

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Access to Document

10.1016/j.aca.2018.11.007