Abstract
A novel real-time polymerase chain reaction (qPCR) platform for the simple and robust detection of platinum is described for the first time. Compared with conventional qPCR, a helper template, which is related to the active template for performing qPCR, was introduced in our helper qPCR system. Several guanine (G) bases were introduced in the helper template to obtain a platinum-responsive on/off switch based on G-Pt-G coordination. Because of the helper template, a slight change in platinum concentration would significantly change the signal in the qPCR. This novel helper qPCR technique easily detects platinum with high sensitivity (1 ng/mL) and selectivity over other metal ions. Therefore, it will be a promising technique for the detection of platinum in biomedical and environmental samples.
| Original language | English |
|---|---|
| Pages (from-to) | 154-160 |
| Number of pages | 7 |
| Journal | Analytica Chimica Acta |
| Volume | 1050 |
| DOIs | |
| Publication status | Published - 2019 Mar 7 |
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Keywords
- Helper qPCR platform
- Platinum detection
- Platinum-responsive on/off switch
- Pt-DNA coordination
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Environmental Chemistry
- Spectroscopy
Cite this
A novel helper qPCR system for platinum detection via Pt-DNA coordination. / Cai, Sheng; Yang, Dan; Tian, Xueke; Ye, Jiawei; Xu, Mingcheng; Abdullah AL-maskri, Abdu Ahmed; Jung, Cheulhee; Zeng, Su.
In: Analytica Chimica Acta, Vol. 1050, 07.03.2019, p. 154-160.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A novel helper qPCR system for platinum detection via Pt-DNA coordination
AU - Cai, Sheng
AU - Yang, Dan
AU - Tian, Xueke
AU - Ye, Jiawei
AU - Xu, Mingcheng
AU - Abdullah AL-maskri, Abdu Ahmed
AU - Jung, Cheulhee
AU - Zeng, Su
PY - 2019/3/7
Y1 - 2019/3/7
N2 - A novel real-time polymerase chain reaction (qPCR) platform for the simple and robust detection of platinum is described for the first time. Compared with conventional qPCR, a helper template, which is related to the active template for performing qPCR, was introduced in our helper qPCR system. Several guanine (G) bases were introduced in the helper template to obtain a platinum-responsive on/off switch based on G-Pt-G coordination. Because of the helper template, a slight change in platinum concentration would significantly change the signal in the qPCR. This novel helper qPCR technique easily detects platinum with high sensitivity (1 ng/mL) and selectivity over other metal ions. Therefore, it will be a promising technique for the detection of platinum in biomedical and environmental samples.
AB - A novel real-time polymerase chain reaction (qPCR) platform for the simple and robust detection of platinum is described for the first time. Compared with conventional qPCR, a helper template, which is related to the active template for performing qPCR, was introduced in our helper qPCR system. Several guanine (G) bases were introduced in the helper template to obtain a platinum-responsive on/off switch based on G-Pt-G coordination. Because of the helper template, a slight change in platinum concentration would significantly change the signal in the qPCR. This novel helper qPCR technique easily detects platinum with high sensitivity (1 ng/mL) and selectivity over other metal ions. Therefore, it will be a promising technique for the detection of platinum in biomedical and environmental samples.
KW - Helper qPCR platform
KW - Platinum detection
KW - Platinum-responsive on/off switch
KW - Pt-DNA coordination
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UR - http://www.scopus.com/inward/citedby.url?scp=85056818537&partnerID=8YFLogxK
U2 - 10.1016/j.aca.2018.11.007
DO - 10.1016/j.aca.2018.11.007
M3 - Article
C2 - 30661583
AN - SCOPUS:85056818537
VL - 1050
SP - 154
EP - 160
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
SN - 0003-2670
ER -