A novel low-molecular weight alkaline mannanase from Streptomyces tendae

Hah Young Yoo, G. C. Pradeep, Seung Wook Kim, Don Hee Park, Yun Hee Choi, Joo Won Suh, Jin Cheol Yoo

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A novel, low-molecular weight, alkaline mannanase from Streptomyces tendae (MnSt) was purified to homogeneity and biochemically characterized. The extracellular mannanase was purified with 26.3% yield using a Sepharose Cl-6B column. The molecular mass of MnSt was approximately 24 kDa. MnSt was stable over a broad pH range (5 ∼ 12.5), was thermally stable at 60°C, and functioned optimally at 50°C and a pH of 12.0. MnSt had K<inf>m</inf> and V<inf>max</inf> values of 0.05 ± 1 mg/mL and 439 ± 0.5mmol/min, respectively, using bean gum galactomannan as a substrate. The N-terminal sequence of MnSt was GWSVDAPYIAXQPFS. Thin layer chromatography (TLC) analysis of the MnSt hydrolysis products suggested that the major oligosaccharide produced was mannobiose. MnSt activity was remarkably affected by metal ions, modulators, chelators, and detergents. MnSt was simple to purify, had high thermal stability, was stable over a broad pH range, and produced mannooligosaccharides. MnSt has high potential for use as an industrial biocatalyst, particularly as a bio-bleaching agent or for oligosaccharide production.

Original languageEnglish
Pages (from-to)453-461
Number of pages9
JournalBiotechnology and Bioprocess Engineering
Volume20
Issue number3
DOIs
Publication statusPublished - 2015 Jun 25

Fingerprint

Oligosaccharides
Streptomyces
Molecular Weight
Molecular weight
Bleaching Agents
Thin layer chromatography
Biocatalysts
Detergents
Molecular mass
Chelating Agents
Bleaching
Sepharose
Modulators
Metal ions
Hydrolysis
Thermodynamic stability
Substrates
Enzymes
Gingiva
Thin Layer Chromatography

Keywords

  • alkaline
  • extracellular mannanase
  • low molecular weight
  • purification

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Biomedical Engineering
  • Bioengineering

Cite this

A novel low-molecular weight alkaline mannanase from Streptomyces tendae. / Yoo, Hah Young; Pradeep, G. C.; Kim, Seung Wook; Park, Don Hee; Choi, Yun Hee; Suh, Joo Won; Yoo, Jin Cheol.

In: Biotechnology and Bioprocess Engineering, Vol. 20, No. 3, 25.06.2015, p. 453-461.

Research output: Contribution to journalArticle

Yoo, Hah Young ; Pradeep, G. C. ; Kim, Seung Wook ; Park, Don Hee ; Choi, Yun Hee ; Suh, Joo Won ; Yoo, Jin Cheol. / A novel low-molecular weight alkaline mannanase from Streptomyces tendae. In: Biotechnology and Bioprocess Engineering. 2015 ; Vol. 20, No. 3. pp. 453-461.
@article{04a8b570be964b89bcc824b1ba8ba34b,
title = "A novel low-molecular weight alkaline mannanase from Streptomyces tendae",
abstract = "A novel, low-molecular weight, alkaline mannanase from Streptomyces tendae (MnSt) was purified to homogeneity and biochemically characterized. The extracellular mannanase was purified with 26.3{\%} yield using a Sepharose Cl-6B column. The molecular mass of MnSt was approximately 24 kDa. MnSt was stable over a broad pH range (5 ∼ 12.5), was thermally stable at 60°C, and functioned optimally at 50°C and a pH of 12.0. MnSt had Km and Vmax values of 0.05 ± 1 mg/mL and 439 ± 0.5mmol/min, respectively, using bean gum galactomannan as a substrate. The N-terminal sequence of MnSt was GWSVDAPYIAXQPFS. Thin layer chromatography (TLC) analysis of the MnSt hydrolysis products suggested that the major oligosaccharide produced was mannobiose. MnSt activity was remarkably affected by metal ions, modulators, chelators, and detergents. MnSt was simple to purify, had high thermal stability, was stable over a broad pH range, and produced mannooligosaccharides. MnSt has high potential for use as an industrial biocatalyst, particularly as a bio-bleaching agent or for oligosaccharide production.",
keywords = "alkaline, extracellular mannanase, low molecular weight, purification",
author = "Yoo, {Hah Young} and Pradeep, {G. C.} and Kim, {Seung Wook} and Park, {Don Hee} and Choi, {Yun Hee} and Suh, {Joo Won} and Yoo, {Jin Cheol}",
year = "2015",
month = "6",
day = "25",
doi = "10.1007/s12257-014-0885-8",
language = "English",
volume = "20",
pages = "453--461",
journal = "Biotechnology and Bioprocess Engineering",
issn = "1226-8372",
publisher = "Korean Society for Biotechnology and Bioengineering",
number = "3",

}

TY - JOUR

T1 - A novel low-molecular weight alkaline mannanase from Streptomyces tendae

AU - Yoo, Hah Young

AU - Pradeep, G. C.

AU - Kim, Seung Wook

AU - Park, Don Hee

AU - Choi, Yun Hee

AU - Suh, Joo Won

AU - Yoo, Jin Cheol

PY - 2015/6/25

Y1 - 2015/6/25

N2 - A novel, low-molecular weight, alkaline mannanase from Streptomyces tendae (MnSt) was purified to homogeneity and biochemically characterized. The extracellular mannanase was purified with 26.3% yield using a Sepharose Cl-6B column. The molecular mass of MnSt was approximately 24 kDa. MnSt was stable over a broad pH range (5 ∼ 12.5), was thermally stable at 60°C, and functioned optimally at 50°C and a pH of 12.0. MnSt had Km and Vmax values of 0.05 ± 1 mg/mL and 439 ± 0.5mmol/min, respectively, using bean gum galactomannan as a substrate. The N-terminal sequence of MnSt was GWSVDAPYIAXQPFS. Thin layer chromatography (TLC) analysis of the MnSt hydrolysis products suggested that the major oligosaccharide produced was mannobiose. MnSt activity was remarkably affected by metal ions, modulators, chelators, and detergents. MnSt was simple to purify, had high thermal stability, was stable over a broad pH range, and produced mannooligosaccharides. MnSt has high potential for use as an industrial biocatalyst, particularly as a bio-bleaching agent or for oligosaccharide production.

AB - A novel, low-molecular weight, alkaline mannanase from Streptomyces tendae (MnSt) was purified to homogeneity and biochemically characterized. The extracellular mannanase was purified with 26.3% yield using a Sepharose Cl-6B column. The molecular mass of MnSt was approximately 24 kDa. MnSt was stable over a broad pH range (5 ∼ 12.5), was thermally stable at 60°C, and functioned optimally at 50°C and a pH of 12.0. MnSt had Km and Vmax values of 0.05 ± 1 mg/mL and 439 ± 0.5mmol/min, respectively, using bean gum galactomannan as a substrate. The N-terminal sequence of MnSt was GWSVDAPYIAXQPFS. Thin layer chromatography (TLC) analysis of the MnSt hydrolysis products suggested that the major oligosaccharide produced was mannobiose. MnSt activity was remarkably affected by metal ions, modulators, chelators, and detergents. MnSt was simple to purify, had high thermal stability, was stable over a broad pH range, and produced mannooligosaccharides. MnSt has high potential for use as an industrial biocatalyst, particularly as a bio-bleaching agent or for oligosaccharide production.

KW - alkaline

KW - extracellular mannanase

KW - low molecular weight

KW - purification

UR - http://www.scopus.com/inward/record.url?scp=84937929222&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84937929222&partnerID=8YFLogxK

U2 - 10.1007/s12257-014-0885-8

DO - 10.1007/s12257-014-0885-8

M3 - Article

VL - 20

SP - 453

EP - 461

JO - Biotechnology and Bioprocess Engineering

JF - Biotechnology and Bioprocess Engineering

SN - 1226-8372

IS - 3

ER -