A novel low-molecular weight alkaline mannanase from Streptomyces tendae

Hah Young Yoo, G. C. Pradeep, Seung Wook Kim, Don Hee Park, Yun Hee Choi, Joo Won Suh, Jin Cheol Yoo

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A novel, low-molecular weight, alkaline mannanase from Streptomyces tendae (MnSt) was purified to homogeneity and biochemically characterized. The extracellular mannanase was purified with 26.3% yield using a Sepharose Cl-6B column. The molecular mass of MnSt was approximately 24 kDa. MnSt was stable over a broad pH range (5 ∼ 12.5), was thermally stable at 60°C, and functioned optimally at 50°C and a pH of 12.0. MnSt had K<inf>m</inf> and V<inf>max</inf> values of 0.05 ± 1 mg/mL and 439 ± 0.5mmol/min, respectively, using bean gum galactomannan as a substrate. The N-terminal sequence of MnSt was GWSVDAPYIAXQPFS. Thin layer chromatography (TLC) analysis of the MnSt hydrolysis products suggested that the major oligosaccharide produced was mannobiose. MnSt activity was remarkably affected by metal ions, modulators, chelators, and detergents. MnSt was simple to purify, had high thermal stability, was stable over a broad pH range, and produced mannooligosaccharides. MnSt has high potential for use as an industrial biocatalyst, particularly as a bio-bleaching agent or for oligosaccharide production.

Original languageEnglish
Pages (from-to)453-461
Number of pages9
JournalBiotechnology and Bioprocess Engineering
Volume20
Issue number3
DOIs
Publication statusPublished - 2015 Jun 25

Keywords

  • alkaline
  • extracellular mannanase
  • low molecular weight
  • purification

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Biomedical Engineering
  • Bioengineering

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