A novel peptide probe for imaging and targeted delivery of liposomal doxorubicin to lung tumor

Xiaofeng He, Moon Hee Na, Jin Sook Kim, Ga Young Lee, Jae Yong Park, Allan S. Hoffman, Ju Ock Nam, Su Eun Han, Ga Yong Sim, Yu Kyoung Oh, In-San Kim, Byung Heon Lee

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Targeted delivery of imaging agents and therapeutics to tumors would provide early detection and increased therapeutic efficacy against cancer. Here we have screened a phage-displayed peptide library to identify peptides that selectively bind to lung tumor cells. Evaluation of individual phage clones after screening revealed that a phage clone displaying the CSNIDARAC peptide bound to H460 lung tumor cells at higher extent than other phage clones. The synthetic CSNIDARAC peptide strongly bound to H460 cells and was efficiently internalized into the cells, while little binding of a control peptide was seen. It also preferentially bound to other lung tumor cell lines as compared to cells of different tumor types. In vivo imaging of lung tumor was achieved by homing of fluorescence dye-labeled CSNIDARAC peptide to the tumor after intravenous injection into mice. Ex vivo imaging and microscopic analysis of isolated organs further demonstrated the targeting of CSNIDARAC peptide to tumor. The CSNIDARAC peptide-targeted and doxorubicin-loaded liposomes inhibited the tumor growth more efficiently than untargeted liposomes or free doxorubicin. In vivo imaging of fluorescence dye-labeled liposomes demonstrated selective homing of the CSNIDARAC-liposomes to tumor. In the same context, higher levels of doxorubicin and apoptosis in tumor tissue were observed when treated with the targeted liposomes than untargeted liposomes or free doxorubicin. These results suggest that the CSNIDARAC peptide is a promising targeting probe that is able to direct imaging agents and therapeutics to lung tumor.

Original languageEnglish
Pages (from-to)430-438
Number of pages9
JournalMolecular Pharmaceutics
Volume8
Issue number2
DOIs
Publication statusPublished - 2011 Apr 4
Externally publishedYes

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Keywords

  • drug delivery
  • imaging
  • liposomal doxorubicin
  • lung tumor
  • peptide probe
  • phage display

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmaceutical Science
  • Drug Discovery

Cite this

He, X., Na, M. H., Kim, J. S., Lee, G. Y., Park, J. Y., Hoffman, A. S., Nam, J. O., Han, S. E., Sim, G. Y., Oh, Y. K., Kim, I-S., & Lee, B. H. (2011). A novel peptide probe for imaging and targeted delivery of liposomal doxorubicin to lung tumor. Molecular Pharmaceutics, 8(2), 430-438. https://doi.org/10.1021/mp100266g