A novel reflectance-based aptasensor using gold nanoparticles for the detection of oxytetracycline

Ho Bin Seo, Young Seop Kwon, Ji Eun Lee, David Cullen, Hongseok Noh, Man Bock Gu

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We present a novel reflectance-based colorimetric aptasensor using gold nanoparticles for the detection of oxytetracycline for the first time. It was found that the reflectance-based measurement at two wavelengths (650 and 520 nm) can generate more stable and sensitive signals than absorbance-based sensors to determine the aggregation of AuNPs, even at high AuNP concentrations. One of the most common antibacterial agents, oxytetracycline (OTC), was detected at concentrations as low as 1 nM in both buffer solution and tap water, which was 25-fold more sensitive, compared to the previous absorbance-based colorimetric aptasensors. This reflectance-based colorimetric aptasensor using gold nanoparticles is considered to be a better platform for portable sensing of small molecules using aptamers.

Original languageEnglish
Pages (from-to)6671-6675
Number of pages5
JournalAnalyst
Volume140
Issue number19
DOIs
Publication statusPublished - 2015 Oct 7

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy
  • Electrochemistry
  • Biochemistry
  • Environmental Chemistry

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