A novel role for thioredoxin reductase in the iron metabolism of S. cerevisiae

Hyun J. Kang, So Woong Choi, Dong Hyuk Heo, Mi Young Jeong, Ha Chin Sung, Cheol-Won Yun

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Intracellular levels of iron are tightly regulated. Saccharomyces cerevisiae uses well-defined pathways to extract iron molecules from the environment. Once inside the cell, the iron molecules must be transferred to target sites via an intracellular iron transporter. Although analogous carriers have been described for other metals, such as copper, an iron transporter has yet to be identified. We used two-dimensional gel electrophoresis and mass spectrometry techniques to attempt to identify the iron transporter from cytosolic fraction of S. cerevisiae. In this study, we identified the iron-binding activity of thioredoxin reductase, and our data suggest a potential role for this enzyme in intracellular iron transport.

Original languageEnglish
Pages (from-to)63-68
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume371
Issue number1
DOIs
Publication statusPublished - 2008 Jun 20

Fingerprint

Thioredoxin-Disulfide Reductase
Metabolism
Saccharomyces cerevisiae
Iron
Yeast
Molecules
Electrophoresis, Gel, Two-Dimensional
Electrophoresis
Mass spectrometry
Copper
Mass Spectrometry
Gels
Metals

Keywords

  • 2-DE
  • IMAC
  • Iron
  • Saccharomyces cerevisiae
  • Thioredoxin reductase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

A novel role for thioredoxin reductase in the iron metabolism of S. cerevisiae. / Kang, Hyun J.; Choi, So Woong; Heo, Dong Hyuk; Jeong, Mi Young; Sung, Ha Chin; Yun, Cheol-Won.

In: Biochemical and Biophysical Research Communications, Vol. 371, No. 1, 20.06.2008, p. 63-68.

Research output: Contribution to journalArticle

Kang, Hyun J. ; Choi, So Woong ; Heo, Dong Hyuk ; Jeong, Mi Young ; Sung, Ha Chin ; Yun, Cheol-Won. / A novel role for thioredoxin reductase in the iron metabolism of S. cerevisiae. In: Biochemical and Biophysical Research Communications. 2008 ; Vol. 371, No. 1. pp. 63-68.
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