A novel vitronectin peptide facilitates differentiation of oligodendrocytes from human pluripotent stem cells (Synthetic ecm for oligodendrocyte differentiation)

Won Ung Park, Gyu Bum Yeon, Myeong Sang Yu, Hui Gwan Goo, Su Hee Hwang, Dokyun Na, Dae Sung Kim

Research output: Contribution to journalArticlepeer-review

Abstract

Differentiation of oligodendrocytes (ODs) presents a challenge in regenerative medicine due to their role in various neurological diseases associated with dysmyelination and demyelination. Here, we designed a peptide derived from vitronectin (VN) using in silico docking simulation and examined its use as a synthetic substrate to support the differentiation of ODs derived from human pluripotent stem cells. The designed peptide, named VNP2, promoted OD differentiation induced by the overexpression of SOX10 in OD precursor cells compared with Matrigel and full-length VN. ODs differentiated on VNP2 exhibited greater contact with axon-mimicking nanofibers than those differentiated on Matrigel. Transcriptomic analysis revealed that the genes associated with morphogenesis, cytoskeleton remodeling, and OD differentiation were upregulated in cells grown on VNP2 compared with cells grown on Matrigel. This new synthetic VN-derived peptide can be used to develop a culture environment for efficient OD differentiation.

Original languageEnglish
Article number1254
JournalBiology
Volume10
Issue number12
DOIs
Publication statusPublished - 2021 Dec

Keywords

  • Differentiation
  • HPSCs
  • Oligodendrocytes
  • Transcriptomic analysis
  • Vitronectin

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Agricultural and Biological Sciences(all)

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