A novel WD40 protein, BnSWD1, is involved in salt stress in Brassica napus

Sanghun Lee, Junhee Lee, Kyung Hee Paek, Suk Yoon Kwon, Hye Sun Cho, Shin Je Kim, Jeong Mee Park

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Genes that are expressed early in specific response to high salinity conditions were isolated from rapeseed plant (Brassica napus L.) using an mRNA differential display method. Five PCR fragments (DD1-5) were isolated that were induced by, but showed different response kinetics to, 200 mM NaCl. Nucleotide sequence analysis and homology search revealed that the deduced amino sequences of three of the five cDNA fragments showed considerable similarity to those of β-mannosidase (DD1), tomato Pti-6 proteins (DD5), and the tobacco harpin-induced protein hin1 (DD4), respectively. In contrast, the remaining clones, DD3 and DD2, did not correspond to any substantial existing annotation. Using the DD3 fragment as a probe, we isolated a full-length cDNA clone from the cDNA library, which we termed BnSWD1 (Brassica napus salt responsive WD40 1). The predicted amino-acid sequence of BnSWD1 contains eight WD40 repeats and is conserved in all eukaryotes. Notably, the BnSWD1 gene is expressed at high levels under salt-stress conditions. Furthermore, we found that BnSWD1 was upregulated after treatment with abscisic acid, salicylic acid, and methyl jasmonate. Our study suggests that BnSWD1, which is a novel WD40 repeat-containing protein, has a function in salt-stress responses in plants, possibly via abscisic acid-dependent and/or -independent signaling pathways.

Original languageEnglish
Pages (from-to)165-172
Number of pages8
JournalPlant Biotechnology Reports
Volume4
Issue number2
DOIs
Publication statusPublished - 2010 Apr 1

Fingerprint

Brassica napus
salt stress
Salts
salts
Proteins
proteins
Abscisic Acid
abscisic acid
mannosidases
clones
Complementary DNA
Clone Cells
Mannosidases
methyl jasmonate
Brassica rapa
rapeseed
Salicylic Acid
sequence homology
salicylic acid
cDNA libraries

Keywords

  • Abscisic acid
  • Brassica napus
  • Brassica napus salt responsive WD40 1gene
  • mRNA differential display
  • Salt stress

ASJC Scopus subject areas

  • Plant Science
  • Biotechnology

Cite this

A novel WD40 protein, BnSWD1, is involved in salt stress in Brassica napus. / Lee, Sanghun; Lee, Junhee; Paek, Kyung Hee; Kwon, Suk Yoon; Cho, Hye Sun; Kim, Shin Je; Park, Jeong Mee.

In: Plant Biotechnology Reports, Vol. 4, No. 2, 01.04.2010, p. 165-172.

Research output: Contribution to journalArticle

Lee, Sanghun ; Lee, Junhee ; Paek, Kyung Hee ; Kwon, Suk Yoon ; Cho, Hye Sun ; Kim, Shin Je ; Park, Jeong Mee. / A novel WD40 protein, BnSWD1, is involved in salt stress in Brassica napus. In: Plant Biotechnology Reports. 2010 ; Vol. 4, No. 2. pp. 165-172.
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