A PCR-RFLP marker distinguishing Ophiostoma clavigerum from morphologically similar Leptographium species associated with bark beetles

Sangwon Lee, Jae-Jin Kim, Simon Fung, Colette Breuil

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Ophiostoma clavigerum, carried by Dendroctonus ponderosae and Dendroctonus jeffreyi, has morphological characteristics that are similar to other Ophiostoma and Leptographium species. The partial β-tubulin gene of 45 strains belonging to seven species was amplified by PCR and digested by the restriction enzyme HinfI. The specific restriction fragment length polymorphism obtained for O. clavigerum provided the means for its reliable identification. We are also reporting that O. clavigerum ascocarps have short necks; this fact has not been shown previously.

Original languageEnglish
Pages (from-to)1104-1112
Number of pages9
JournalCanadian Journal of Botany
Volume81
Issue number11
DOIs
Publication statusPublished - 2003 Nov 1
Externally publishedYes

Fingerprint

Leptographium
Brickellia
Ophiostoma
bark beetles
bark
beetle
restriction fragment length polymorphism
Dendroctonus jeffreyi
Dendroctonus ponderosae
polymorphism
ascomata
enzyme
tubulin
neck
gene
enzymes
marker
genes

Keywords

  • β-tubulin gene
  • Bark beetle
  • Leptographium
  • Ophiostoma clavigerum
  • RFLP

ASJC Scopus subject areas

  • Plant Science

Cite this

A PCR-RFLP marker distinguishing Ophiostoma clavigerum from morphologically similar Leptographium species associated with bark beetles. / Lee, Sangwon; Kim, Jae-Jin; Fung, Simon; Breuil, Colette.

In: Canadian Journal of Botany, Vol. 81, No. 11, 01.11.2003, p. 1104-1112.

Research output: Contribution to journalArticle

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