A poly(lactic acid)/calcium metaphosphate composite for bone tissue engineering

Youngmee Jung, Sang Soo Kim, Ha Kim Young, Sang Heon Kim, Byung Soo Kim, Sukyoung Kim, Yong Choi Cha, Soo Hyun Kim

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

A new method to prepare PLA/CMP (poly-l-lactide/calcium metaphosphate) composite scaffolds was developed for effective bone tissue engineering. This novel sintering method is composed of pressing the mixture of PLA, CMP, and salt particles at 150 MPa for 3 min followed by heat treatment at 210°C for 30 min. The scaffolds had a homogeneously interconnected porous structure without a skin layer, and they exhibited a narrower pore size distribution and higher mechanical strength in comparison with scaffolds made by a solvent casting method. The scaffolds were seeded by osteoblasts and cultured in vitro or implanted into nude mice subcutaneously for up to 5 weeks. The number of cells attached to and proliferated on the scaffolds at both in vitro and in vivo was in the order of; PLA by novel sintering<PLA/CMP by solvent casting<PLA/CMP by novel sintering. In addition, the alkaline phosphatase activity of and calcium deposition in the scaffolds explanted from mice were enhanced significantly for the scaffolds by novel sintering compared to them by solvent casting. The in vitro results agreed well with the in vivo data. Such a superior characteristic of the novel sintering method should have resulted from the fact that the CMP particles could contact directly with cells/tissues to stimulate the cell proliferation and osteogenic differentiation, while the CMP particles would be coated by polymers and hindered to interact with cells/tissues in the case of a solvent casting method. As the novel sintering method does not use any solvents it offers another advantage to avoid problems associated with solvent residue.

Original languageEnglish
Pages (from-to)6314-6322
Number of pages9
JournalBiomaterials
Volume26
Issue number32
DOIs
Publication statusPublished - 2005 Nov 1
Externally publishedYes

Fingerprint

Calcium Carbonate
Lactic acid
Tissue Engineering
Tissue engineering
Scaffolds
Calcium
Bone
Sintering
Cytidine Monophosphate
Bone and Bones
Composite materials
Casting
Tissue
Osteoblasts
Phosphatases
Cell proliferation
Scaffolds (biology)
Nude Mice
Pore size
Strength of materials

Keywords

  • Bone regeneration
  • Calcium metaphosphate
  • Composites
  • Poly-l-lactide
  • Tissue engineering

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

Jung, Y., Kim, S. S., Young, H. K., Kim, S. H., Kim, B. S., Kim, S., ... Kim, S. H. (2005). A poly(lactic acid)/calcium metaphosphate composite for bone tissue engineering. Biomaterials, 26(32), 6314-6322. https://doi.org/10.1016/j.biomaterials.2005.04.007

A poly(lactic acid)/calcium metaphosphate composite for bone tissue engineering. / Jung, Youngmee; Kim, Sang Soo; Young, Ha Kim; Kim, Sang Heon; Kim, Byung Soo; Kim, Sukyoung; Cha, Yong Choi; Kim, Soo Hyun.

In: Biomaterials, Vol. 26, No. 32, 01.11.2005, p. 6314-6322.

Research output: Contribution to journalArticle

Jung, Y, Kim, SS, Young, HK, Kim, SH, Kim, BS, Kim, S, Cha, YC & Kim, SH 2005, 'A poly(lactic acid)/calcium metaphosphate composite for bone tissue engineering', Biomaterials, vol. 26, no. 32, pp. 6314-6322. https://doi.org/10.1016/j.biomaterials.2005.04.007
Jung, Youngmee ; Kim, Sang Soo ; Young, Ha Kim ; Kim, Sang Heon ; Kim, Byung Soo ; Kim, Sukyoung ; Cha, Yong Choi ; Kim, Soo Hyun. / A poly(lactic acid)/calcium metaphosphate composite for bone tissue engineering. In: Biomaterials. 2005 ; Vol. 26, No. 32. pp. 6314-6322.
@article{79f3868eee7242eab8a0af99b896ffd3,
title = "A poly(lactic acid)/calcium metaphosphate composite for bone tissue engineering",
abstract = "A new method to prepare PLA/CMP (poly-l-lactide/calcium metaphosphate) composite scaffolds was developed for effective bone tissue engineering. This novel sintering method is composed of pressing the mixture of PLA, CMP, and salt particles at 150 MPa for 3 min followed by heat treatment at 210°C for 30 min. The scaffolds had a homogeneously interconnected porous structure without a skin layer, and they exhibited a narrower pore size distribution and higher mechanical strength in comparison with scaffolds made by a solvent casting method. The scaffolds were seeded by osteoblasts and cultured in vitro or implanted into nude mice subcutaneously for up to 5 weeks. The number of cells attached to and proliferated on the scaffolds at both in vitro and in vivo was in the order of; PLA by novel sintering<PLA/CMP by solvent casting<PLA/CMP by novel sintering. In addition, the alkaline phosphatase activity of and calcium deposition in the scaffolds explanted from mice were enhanced significantly for the scaffolds by novel sintering compared to them by solvent casting. The in vitro results agreed well with the in vivo data. Such a superior characteristic of the novel sintering method should have resulted from the fact that the CMP particles could contact directly with cells/tissues to stimulate the cell proliferation and osteogenic differentiation, while the CMP particles would be coated by polymers and hindered to interact with cells/tissues in the case of a solvent casting method. As the novel sintering method does not use any solvents it offers another advantage to avoid problems associated with solvent residue.",
keywords = "Bone regeneration, Calcium metaphosphate, Composites, Poly-l-lactide, Tissue engineering",
author = "Youngmee Jung and Kim, {Sang Soo} and Young, {Ha Kim} and Kim, {Sang Heon} and Kim, {Byung Soo} and Sukyoung Kim and Cha, {Yong Choi} and Kim, {Soo Hyun}",
year = "2005",
month = "11",
day = "1",
doi = "10.1016/j.biomaterials.2005.04.007",
language = "English",
volume = "26",
pages = "6314--6322",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier BV",
number = "32",

}

TY - JOUR

T1 - A poly(lactic acid)/calcium metaphosphate composite for bone tissue engineering

AU - Jung, Youngmee

AU - Kim, Sang Soo

AU - Young, Ha Kim

AU - Kim, Sang Heon

AU - Kim, Byung Soo

AU - Kim, Sukyoung

AU - Cha, Yong Choi

AU - Kim, Soo Hyun

PY - 2005/11/1

Y1 - 2005/11/1

N2 - A new method to prepare PLA/CMP (poly-l-lactide/calcium metaphosphate) composite scaffolds was developed for effective bone tissue engineering. This novel sintering method is composed of pressing the mixture of PLA, CMP, and salt particles at 150 MPa for 3 min followed by heat treatment at 210°C for 30 min. The scaffolds had a homogeneously interconnected porous structure without a skin layer, and they exhibited a narrower pore size distribution and higher mechanical strength in comparison with scaffolds made by a solvent casting method. The scaffolds were seeded by osteoblasts and cultured in vitro or implanted into nude mice subcutaneously for up to 5 weeks. The number of cells attached to and proliferated on the scaffolds at both in vitro and in vivo was in the order of; PLA by novel sintering<PLA/CMP by solvent casting<PLA/CMP by novel sintering. In addition, the alkaline phosphatase activity of and calcium deposition in the scaffolds explanted from mice were enhanced significantly for the scaffolds by novel sintering compared to them by solvent casting. The in vitro results agreed well with the in vivo data. Such a superior characteristic of the novel sintering method should have resulted from the fact that the CMP particles could contact directly with cells/tissues to stimulate the cell proliferation and osteogenic differentiation, while the CMP particles would be coated by polymers and hindered to interact with cells/tissues in the case of a solvent casting method. As the novel sintering method does not use any solvents it offers another advantage to avoid problems associated with solvent residue.

AB - A new method to prepare PLA/CMP (poly-l-lactide/calcium metaphosphate) composite scaffolds was developed for effective bone tissue engineering. This novel sintering method is composed of pressing the mixture of PLA, CMP, and salt particles at 150 MPa for 3 min followed by heat treatment at 210°C for 30 min. The scaffolds had a homogeneously interconnected porous structure without a skin layer, and they exhibited a narrower pore size distribution and higher mechanical strength in comparison with scaffolds made by a solvent casting method. The scaffolds were seeded by osteoblasts and cultured in vitro or implanted into nude mice subcutaneously for up to 5 weeks. The number of cells attached to and proliferated on the scaffolds at both in vitro and in vivo was in the order of; PLA by novel sintering<PLA/CMP by solvent casting<PLA/CMP by novel sintering. In addition, the alkaline phosphatase activity of and calcium deposition in the scaffolds explanted from mice were enhanced significantly for the scaffolds by novel sintering compared to them by solvent casting. The in vitro results agreed well with the in vivo data. Such a superior characteristic of the novel sintering method should have resulted from the fact that the CMP particles could contact directly with cells/tissues to stimulate the cell proliferation and osteogenic differentiation, while the CMP particles would be coated by polymers and hindered to interact with cells/tissues in the case of a solvent casting method. As the novel sintering method does not use any solvents it offers another advantage to avoid problems associated with solvent residue.

KW - Bone regeneration

KW - Calcium metaphosphate

KW - Composites

KW - Poly-l-lactide

KW - Tissue engineering

UR - http://www.scopus.com/inward/record.url?scp=20444456153&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=20444456153&partnerID=8YFLogxK

U2 - 10.1016/j.biomaterials.2005.04.007

DO - 10.1016/j.biomaterials.2005.04.007

M3 - Article

VL - 26

SP - 6314

EP - 6322

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 32

ER -