A Portable Diagnostic Assay, Genetic Diversity, and Isolation of Seoul Virus from Rattus norvegicus Collected in Gangwon Province, Republic of Korea

Kyungmin Park, Seung Ho Lee, Jongwoo Kim, Jingyeong Lee, Geum Young Lee, Seungchan Cho, Juyoung Noh, Jeewan Choi, Juwon Park, Dong Hyun Song, Se Hun Gu, Hyeongseok Yun, Jung Eun Kim, Daesang Lee, Il Ung Hwang, Won Keun Kim, Jin Won Song

Research output: Contribution to journalArticlepeer-review

Abstract

Seoul virus (SEOV), an etiological agent for hemorrhagic fever with renal syndrome, poses a significant public health threat worldwide. This study evaluated the feasibility of a mobile Biomeme platform for facilitating rapid decision making of SEOV infection. A total of 27 Rattus norvegicus were collected from Seoul Metropolitan City and Gangwon Province in Republic of Korea (ROK), during 2016–2020. The serological and molecular prevalence of SEOV was 5/27 (18.5%) and 2/27 (7.4%), respectively. SEOV RNA was detected in multiple tissues of rodents using the Biomeme device, with differences in Ct values ranging from 0.6 to 2.1 cycles compared to a laboratory benchtop system. Using amplicon-based next-generation sequencing, whole-genome sequences of SEOV were acquired from lung tissues of Rn18-1 and Rn19-5 collected in Gangwon Province. Phylogenetic analysis showed a phylogeographical diversity of rat-borne orthohantavirus collected in Gangwon Province. We report a novel isolate of SEOV Rn19-5 from Gangwon Province. Our findings demonstrated that the Biomeme system can be applied for the molecular diagnosis of SEOV comparably to the laboratory-based platform. Whole-genome sequencing of SEOV revealed the phylogeographical diversity of orthohantavirus in the ROK. This study provides important insights into the field-deployable diagnostic assays and genetic diversity of orthohantaviruses for the rapid response to hantaviral outbreaks in the ROK.

Original languageEnglish
Article number1047
JournalPathogens
Volume11
Issue number9
DOIs
Publication statusPublished - 2022 Sep

Keywords

  • Biomeme system
  • genetic diversity
  • hantavirus isolation
  • hemorrhagic fever with renal syndrome
  • molecular diagnosis
  • next-generation sequencing
  • portable diagnostic assay
  • Seoul virus
  • whole-genome sequencing

ASJC Scopus subject areas

  • Immunology and Allergy
  • Molecular Biology
  • Immunology and Microbiology(all)
  • Microbiology (medical)
  • Infectious Diseases

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