A possible role of kainate receptors in C2C12 skeletal myogenic cells

Jae-Yong Park, Jaehee Han, Seong Geun Hong

Research output: Contribution to journalArticle

Abstract

Ca 2+ influx appears to be important for triggering myoblast fusion. It remains, however, unclear how Ca 2+ influx rises prior to myoblast fusion. Recently, several studies suggested that NMDA receptors may be involved in Ca 2+ mobilization of muscle, and that Ca 2+ influx is mediated by NMDA receptors in C2C12 myoblasts. Here, we report that other types of ionotropic glutamate receptors, non-NMDA receptors (AMPA and KA receptors), are also involved in Ca 2+ influx in myoblasts. To explore which subtypes of non-NMDA receptors are expressed in C2C12 myogenic cells, RT-PCR was performed, and the results revealed that KA receptor subunits were expressed in both myoblasts and myotubes. However, AMPA receptor was not detected in myoblasts but expressed in myotubes. Using a Ca 2+ imaging system, Ca 2+ influx mediated by these receptors was directly measured in a single myoblast cell. Intracellular Ca 2+ level was increased by KA, but not by AMPA. These results were consistent with RT-PCR data. In addition, KA-induced intracellular Ca 2+ increase was completely suppressed by treatment of nifedifine, a L-type Ca 2+ channel blocker. Furthermore, KA stimulated myoblast fusion in a dose-dependent manner. CNQX inhibited not only KA-induced myoblast fusion but also spontaneous myoblast fusion. Therefore, these results suggest that KA receptors are involved in intracellular Ca 2+ increase in myoblasts and then may play an important role in myoblast fusion.

Original languageEnglish
Pages (from-to)375-379
Number of pages5
JournalKorean Journal of Physiology and Pharmacology
Volume7
Issue number6
Publication statusPublished - 2003 Dec 1
Externally publishedYes

Fingerprint

Kainic Acid Receptors
Myoblasts
AMPA Receptors
Skeletal Muscle Fibers
N-Methyl-D-Aspartate Receptors
6-Cyano-7-nitroquinoxaline-2,3-dione
Ionotropic Glutamate Receptors
Polymerase Chain Reaction
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid

Keywords

  • AMPA receptors
  • Ca influx
  • KA receptors
  • Myoblast fusion

ASJC Scopus subject areas

  • Physiology
  • Pharmacology

Cite this

A possible role of kainate receptors in C2C12 skeletal myogenic cells. / Park, Jae-Yong; Han, Jaehee; Hong, Seong Geun.

In: Korean Journal of Physiology and Pharmacology, Vol. 7, No. 6, 01.12.2003, p. 375-379.

Research output: Contribution to journalArticle

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