A practical enzymatic synthesis of UDP sugars and NDP glucoses

Jungdon Bae, Kwang Ho Kim, Dooil Kim, Yongseok Choi, Joong Su Kim, Sukhoon Koh, Suk In Hong, Dae Sil Lee

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Mix and match. Nucleotide sugars are essential as glycoside donors to glycosyltransferases for the synthesis of bioactive metabolites. With 45 combinations of reactions with five NTPs and nine sugar-1-phosphates (Su1Ps) by recombinant UDP-sugar pyrophosphorylase (Up) from Thermus caldophilus GK24, the enzyme showed broad substrate specificity toward five types of NTP with glucose-1-phosphate as well as four Su1Ps with UTP, affording all nine nucleotide sugars.

Original languageEnglish
Pages (from-to)1963-1966
Number of pages4
JournalChemBioChem
Volume6
Issue number11
DOIs
Publication statusPublished - 2005 Nov 1
Externally publishedYes

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Keywords

  • Enzyme catalysis
  • Glycosyltransferases
  • Nucleotide sugars
  • Thermus caldophilus

ASJC Scopus subject areas

  • Drug Discovery
  • Organic Chemistry
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Bae, J., Kim, K. H., Kim, D., Choi, Y., Kim, J. S., Koh, S., Hong, S. I., & Lee, D. S. (2005). A practical enzymatic synthesis of UDP sugars and NDP glucoses. ChemBioChem, 6(11), 1963-1966. https://doi.org/10.1002/cbic.200500183