A practical enzymatic synthesis of UDP sugars and NDP glucoses

Jungdon Bae, Kwang Ho Kim, Dooil Kim, Yongseok Choi, Joong Su Kim, Sukhoon Koh, Suk In Hong, Dae Sil Lee

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Mix and match. Nucleotide sugars are essential as glycoside donors to glycosyltransferases for the synthesis of bioactive metabolites. With 45 combinations of reactions with five NTPs and nine sugar-1-phosphates (Su1Ps) by recombinant UDP-sugar pyrophosphorylase (Up) from Thermus caldophilus GK24, the enzyme showed broad substrate specificity toward five types of NTP with glucose-1-phosphate as well as four Su1Ps with UTP, affording all nine nucleotide sugars.

Original languageEnglish
Pages (from-to)1963-1966
Number of pages4
JournalChemBioChem
Volume6
Issue number11
DOIs
Publication statusPublished - 2005 Nov 1
Externally publishedYes

Fingerprint

Uridine Diphosphate Sugars
Sugar Phosphates
Sugars
Nucleotides
Thermus
Glucose
Glycosyltransferases
Uridine Triphosphate
Glycosides
Substrate Specificity
Phosphates
Enzymes
Metabolites
Substrates

Keywords

  • Enzyme catalysis
  • Glycosyltransferases
  • Nucleotide sugars
  • Thermus caldophilus

ASJC Scopus subject areas

  • Drug Discovery
  • Organic Chemistry
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Bae, J., Kim, K. H., Kim, D., Choi, Y., Kim, J. S., Koh, S., ... Lee, D. S. (2005). A practical enzymatic synthesis of UDP sugars and NDP glucoses. ChemBioChem, 6(11), 1963-1966. https://doi.org/10.1002/cbic.200500183

A practical enzymatic synthesis of UDP sugars and NDP glucoses. / Bae, Jungdon; Kim, Kwang Ho; Kim, Dooil; Choi, Yongseok; Kim, Joong Su; Koh, Sukhoon; Hong, Suk In; Lee, Dae Sil.

In: ChemBioChem, Vol. 6, No. 11, 01.11.2005, p. 1963-1966.

Research output: Contribution to journalArticle

Bae, J, Kim, KH, Kim, D, Choi, Y, Kim, JS, Koh, S, Hong, SI & Lee, DS 2005, 'A practical enzymatic synthesis of UDP sugars and NDP glucoses', ChemBioChem, vol. 6, no. 11, pp. 1963-1966. https://doi.org/10.1002/cbic.200500183
Bae, Jungdon ; Kim, Kwang Ho ; Kim, Dooil ; Choi, Yongseok ; Kim, Joong Su ; Koh, Sukhoon ; Hong, Suk In ; Lee, Dae Sil. / A practical enzymatic synthesis of UDP sugars and NDP glucoses. In: ChemBioChem. 2005 ; Vol. 6, No. 11. pp. 1963-1966.
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